Objective To investigate the relationship between perihematomal edema (PHE) and changes in the polarity of aquaporin-4 (AQP4) following intracerebral hemorrhage (ICH) in mice. Methods Eighteen C57BL/6 mice were randomly divided into the sham-operation group (Sham group) and the 24-hour post-ICH group (ICH group). PHE was observed using MR T2-weighted imaging (T2WI). The changes in astrocytic endfeet were observed by transmission electron microscopy (TEM),and the expression of AQP4-M23 was detected by western blot to reflect the degree of AQP4 polarization. Additionally,immunofluorescence staining was used to assess the colocalization of AQP4 with CD31 (a vascular-specific marker) to reveal the degree of AQP4 polarization. Statistical analysis was performed using GraphPad Prism 9.0 software. Quantitative data that met the normal distribution and homogeneity of variance are presented as (x)±s,and comparisons between the two groups were made using the t-test,with P<0.05 considered statistically significant. Results On the T2WI sequence,the ICH group showed a patchy hypointense signal in the basal ganglia region,surrounded by an irregular hyperintense signal,indicating significant PHE after intracerebral hemorrhage. TEM examination revealed extensive swelling of astrocytic endfeet around blood vessels in the PHE region. Western blot analysis showed a significant reduction in AQP4-M23 expression compared to the Sham group (t=3.892,P<0.01). Immunofluorescence images of the brain tissue in the PHE region showed a significant reduction in the colocalization of AQP4 with the vascular marker CD31,indicating decreased AQP4 polarization. Conclusion The formation of PHE in mice following intracerebral hemorrhage is closely related to the decreased polarization of AQP4.
维普
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