首页|抗氧化肽AOP1对小鼠皮肤烫伤创面愈合修复的影响

抗氧化肽AOP1对小鼠皮肤烫伤创面愈合修复的影响

Effect of antioxidant peptide AOP1 on healing repair of skin burns in mice

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  • 国家科技期刊平台
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目的 探究抗氧化肽AOP1促进小鼠皮肤烫伤创面愈合修复的作用及机制.方法 选用DCFH-DA检测细胞内活性氧(ROS)的变化;通过细胞增殖与迁移实验检测药物毒性及对创面愈合的影响.采用直径1 cm铜柱80℃水浴2min、紧贴小鼠皮肤10 s的方法制备烫伤模型,观察烫伤创面愈合情况;并用HE和Masson三色染色观察AOP1对皮肤烫伤创面愈合修复的作用;检测小鼠全皮组织中MDA含量和SOD活性.结果 在细胞水平上,抗氧化肽AOP1能够明显降低HaCaT和L929细胞内ROS的含量,促进细胞迁移和增殖.与对照组相比,AOP1组小鼠皮肤创面愈合时间短、愈合率高,结痂面积小,可明显降低烫伤创伤组织中炎症反应及MDA的含量;HE和Masson染色也证实了AOP1对烫伤创面愈合修复的促进作用.结论 具有天然活性的抗氧化肽AOP1可能通过减轻烫伤引起的氧化应激,促进小鼠皮肤烫伤创面的愈合修复.
Aim To explore the effect and mechanism of antioxidant peptide AOP1 on repair of skin burn wound healing in mice.Methods Fluorescence probe DCFH-DA was used to detect the changes of intracellular reactive oxygen species (ROS).Cell proliferation and migration assay were used to detect AOP1 toxicity and its effect on wound healing.Moreover,the skin scald wound was made on the shaved dorsum of the anesthetized mice with a 1.0 cm diameter brass cylinder heated in a water bath at 80 ℃ for 2 min and pressed against the rat skin for 10 s.The effects of AOP1 on the healing of skin burns were observed by HE and Masson staining and the contents of MDA and the activity of SOD in skin tissues were measured.Results The antioxidant peptide AOP1 could significantly reduce the number of ROS in HaCaT and L929 cells,and promote cell migration and proliferation.Compared with the untreated group,the skin healing time of AOP1 group was short,the healing rate was high,the area of scab was small,and inflammation and the content of MDA in burned tissue significantly decreased.The effect of AOP1 on healing of burn wound healing was also confirmed by HE and Masson staining.Conclusion It is suggested that the antioxidant peptide AOP1 with natural activity may promote the healing of skin burns by reducing the oxidative stress caused by burns.

antioxidant peptideAOP1skin burnswound repairoxidative stressinflammation

秦迪、张慧杰、杨希营、陈艳通、林维平、李文辉、孙同毅、高媛媛

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潍坊医学院生物科学与技术学院,山东省高校生物药物重点实验室,山东潍坊261053

潍坊医学院麻醉学系,山东潍坊261053

潍坊医学院药学院,山东潍坊261053

抗氧化肽 AOP1 皮肤烫伤 创面修复 氧化应激 炎症反应

国家自然科学基金山东省自然科学基金山东省自然科学基金山东省高等学校科技计划项目山东省医药卫生科技发展计划项目山东省医药卫生科技发展计划项目潍坊医学院教师公派教师国内访学项目经费

31600775ZR2015HL056ZR2016HM76J15LM102015WS00582015WS0059

2018

10.3969/j.issn.1001-1978.2018.02.016

中国药理学通报
中国药理学会

中国药理学通报

CSTPCDCSCD北大核心
影响因子：1.54
ISSN：1001-1978
年,卷(期)：2018.34(2)
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