Effect of Liuwei Dihuang decoction on β-catenin and EMT in kidney tissue of UUO rats
Aim To explore the effect of Liuwei Di-huang decoction(LWDHD)on the expression of β-catenin,E-cadherin,α-SMA,the pathological changes of renal tissue,and the changes of an epithelial-mesen-chymal transformation(EMT)in renal tissue of rats with unilateral ureteral obstruction(UUO).Methods Forty-eight SPF grade SD rats were randomly divided into sham group(Sham),model group(UUO),Li-uwei Dihuang decoction low,medium,and high groups(LWDHD 3.375,6.75,13.5 g·kg-1)and enalapril group(10 mg·kg-1),with eight rats in each group.HE and Masson staining were used to observe the path-ological changes and collagen fiber deposition in the af-fected renal tissue of the six groups.Immunohisto-chemistry and Western blot were employed to detect the expression of E-cadherin,β-catenin,and α-SMA in re-nal tissue of rats in each group.Results Compared with the sham group,the UUO group showed glomeru-lar sclerosis,tubular dilatation,and more severe inter-stitial fibrosis.The expression of β-catenin and α-SMA protein increased,while the expression of E-cadherin protein decreased(P＜0.05).Compared with the UUO group,the pathological changes and collagen deposition of renal tissue in the LWDHD group and en-alapril group were significantly improved,the protein levels of β-catenin and α-SMA decreased,while the protein level of E-cadherin increased(P＜0.05).Conclusions LiuWei Dihuang decoction may down-regulate β-catenin expression,and then inhibit EMT,thus playing a role in alleviating renal fibrosis.
国家科技期刊平台
NSTL
万方数据
维普
