A study on comprehensive quality evaluation of six batches of Rhizoma Pinelliae for protective effects on chronic obstructive pulmonary disease based on TOPSIS method
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维普
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目的 通过离散型逼近理想解排序法(technique for order preference by similarity to an ideal solution,TOPSIS)评估6批法半夏醇提物的体外抗慢性阻塞性肺疾病(chronic obstructive pulmonary disease,COPD)作用,建立法半夏综合品质评价新方法.方法 采用香烟烟雾提取物(cigarette smoke extract,CSE)和脂多糖(lipopolysaccharide,LPS)构建Beas-2b人正常肺细胞的COPD模型,并使用MTT法测定生半夏及6批法半夏醇提取物的毒性和药效浓度,同时计算EC50、IC50;再由 qRT-PCR、超氧化物歧化酶(superoxide dis-mutase,SOD)试剂盒测定相关指标;最后通过TOPSIS法对生半夏和法半夏进行多指标综合评价.结果 根据IC50值,得出各批次样品如下毒性排序:生半夏＞法半夏④＞法半夏①＞法半夏⑤＞法半夏③＞法半夏⑥＞法半夏②.通过qRT-PCR法验证得出,与模型组对比,各半夏给药组的TNF-a、IL-6和IL-8的表达均有不同程度的下调;并且在给药后所有给药组的SOD的含量均降低.将所有数据进行归一化处理,通过TOPSIS分析,得到药效综合排序结果如下:法半夏⑥＞法半夏⑤＞法半夏②＞法半夏①＞法半夏③＞法半夏④＞生半夏.结论 不同半夏醇提物均可抑制LPS和CSE诱导的炎症和氧化应激,综合评价第⑥批法半夏的质量最好,且TOPSIS法可以作为一种药材的综合品质评定方法,为市面上参差不齐的药材质量提供质控思路.
Aim To evaluate the in vitro anti-chronic obstructive pulmonary disease(COPD)effects of six batches of Rhizoma Pinelliae(RP)ethanol extracts by the technique for order preference by similarity to an i-deal solution(TOPSIS),so as to establish a new method for comprehensive quality evaluation of RP.Methods Cigarette smoke extract(CSE)and lipopo-lysaccharide(LPS)were utilized to establish an airway inflammation model of Beas-2b human normal lung cells.MTT assay was employed to determine the toxici-ty and pharmacological concentrations of Pinellia Ter-nata(PT)and six different batches RP of their ethanol extracts.EC50 and IC50 values were calculated accord-ingly.Additionally,relevant markers were assessed u-sing qRT-PCR and the superoxide dismutase(SOD)assay kit.Finally,a comprehensive evaluation invol-ving multiple indices was performed using the TOPSIS method for both PT and the six batches of RP extracts.Results According to the IC50 values of seven differ-ent Pinellia ternata,the toxicity ranking was as fol-lows:PT＞RP④＞RP①＞RP⑤＞RP③＞RP⑥＞RP ②.On the other hand,the expressions of TNF-α,IL-6 and IL-8 were down-regulated in the treatment group compared with those of the model group by qRT-PCR.In addition,the levels of SOD de-creased in all groups after treatment.All the data were normalized and the comprehensive ranking results were obtained through TOPSIS analysis as follows:RP⑥＞RP⑤＞RP②＞RP①＞RP③＞RP④＞PT.Con-clusions The ethanol extracts of different Rhizoma Pinelliae varieties all demonstrate the capacity to sup-press inflammation and oxidative stress induced by LPS and CSE.The comprehensive evaluation indicates that the quality of the ⑥ batch of RP shows the highest quality.Moreover,the TOPSIS method can serve as an assessment approach for medicinal materials,offering a pathway for controling the varying quality of herbal ma-terials available in the market.
维普
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