首页|CRISPR/Cas9-mediated knockout of E4 gene promotes maturation in soybean

CRISPR/Cas9-mediated knockout of E4 gene promotes maturation in soybean

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Soybean is a broadly popular and extensively cultivated crop,however,many high-yield and high-quality vari-eties require specific growth conditions,restricting their widespread adoption.The appropriate light conditions and photoperiod must be attained for these varieties to thrive in new environments.In this study,we employed CRISPR/Cas9 to design two sgRNAs aimed at knocking out the maturity-related gene E4 in a major American soybean variety called"Jack",which belongs to maturity group MGⅡ.E4 gene is primarily involved in the photoperiodic flowering and maturity in soybean,making it an ideal candidate for genetic manipulation.We successfully obtained 1 homozygous E4-SG1 mutant type with 1-bp insertion,and 4 homozygous E4-SG2 mutants type with 2-bp deletion,7-bp deletion,61-bp deletion,and 1-bp insertion,respectively.The homozygous e4 mutant plants contained early termination codons devoid of transgenic elements.Additionally,no potential off-target sites of the E4 gene were detected.A comparative analysis revealed that,unlike the wild-type,the maturity time of homozygous e4 mutants was early under both short-day and long-day conditions.These mutants offer novel germplasm resources that may be used to modify the photoperiod sensitivity and maturity of soybean,enhancing its adaptability to high-latitude regions.

SoybeanE4CRISPR/Cas9Maturity

Shuiqing Wu、Li Chen、Mengwei Guo、Yupeng Cai、Yang Gao、Shan Yuan、Shi Sun、Yuxian Zhang、Wensheng Hou、Tianfu Han

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College of Agriculture,Heilongjiang Bayi Agricultural University,Daqing,163316,China

Ministry of Agriculture Key Laboratory of Soybean Biology(Beijing),Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing,100081,China

Ministry of Agriculture Key Laboratory of Soybean Biology(Beijing),Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 100081,China

College of Agriculture,Northeast Agricultural University,Harbin 150030,China

State Key Laboratory of Crop Germplasm Innovation and Molecular Breeding,Syngenta Biotechnology(China)Co.,Ltd,Beijing,100081,China

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2024

10.1016/j.ocsci.2024.05.001

中国油料作物学报(英文)
中国农业科学院油料作物研究所

中国油料作物学报(英文)

ISSN：2096-2428
年,卷(期)：2024.9(3)