Determination of residual EDTA-2Na in Japanese encephalitis attenuated live vaccine by capillary electrophoresis with electrophoretically mediated microanalysis
Objective:To establish a capillary electrophoresis(CE)with electrophoretically mediated microanaly-sis(EMMA)method for the determination of EDTA-2Na in Japanese encephalitis attenuated live vaccine.Methods:The test was performed in disodium hydrogen phosphate buffer with pH 2.5,the online metal ions com-plexation of 1.5 mg·mL-1 Fe3+and incubation time of 3 min.The separation voltage was 25 kV,the detection wavelength was 257 nm,and.the column temperature was 25.0 ℃.Results:The established method had a good linear relationship in the concentration range of 0.01-0.5 mg·mL-1(r=0.999 9),the detection limit was 5 μg·mL-1,and the relative standard deviation(RSD)of the measured samples was less than 2.87％.The recoveries of spiked samples were between 96.49％-101.02％.Conclusion:The optimized method was applied to the determination of EDTA-2Na in Japanese encephalitis attenuated live vaccine.The satisfactory experimental results were obtained.
capillary electrophoresiselectrophoretically mediated microanalysisJapanese encephalitis attenuated live vaccineEDTA-2Naresidual quantityquality control
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