Simultaneous determination of four diterpene lactones in Lianzhi Xiaoyan capsules by QAMS
Objective:To set up a multi-components by single maker(QAMS)method for determination of four diterpene lactones in Lianzhi Xiaoyan capsules.Methods:Shimadzu Shim-pack C18 column(250 mm ×4.6 mm,5μm)was adopted with 35 ℃.Acetonitrile-0.1％phosphoric acid aqueous solution as the mobile phase in gradient mode was used at flow rateof 1.0 mL·min-1.The detection wavelength was set at 203 nm.Andrographolide was used as reference to calculate the relative correction factors of the other three components.Results:The linear rela-tionships of four components were good(r ≥ 0.999 9)within their ranges,and their average recoveries were 100.0％-102.8％with RSDs 1.1％-2.2％.The results obtained by QAMS were close to those obtained by ex-ternal standard method.Conclusion:This rapid,precise and reproducible method can be used for the quality con-trol of Lianzhi Xiaoyan capsules.
Lianzhi Xiaoyan capsulesandrographolidediterpene lactonecorrection factorQAMSinternal standard method
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