Simultaneous determination of calceolarioside B and chlorogenic acid in Caulis Stauntoniae Chinensis tablets by HPLC
Objective:To establish a method for simultaneous determination of calceolarioside B and chlorogenic acid in Caulis Stauntoniae Chinensis tablets by HPLC.Methods:The analysis was performed on a Thermo BDS HYPERSIL C18 column(4.6 mm ×250 mm,5 μm)maintained at 35 ℃.The mobile phase was consisted of meth-anol and 0.1％phosphoric acid solution,and gradient eluted with a flow rate of 1.0 mL·min-1.The detection wavelength was 327 nm,and the injection volume was 10 μL.Results:The linear ranges of calceolarioside B and chlorogenic acid were 0.51-20.60 μg·mL-1(r=1.000)and 0.52-20.63 μg·mL-1(r=1.000),respective-ly.The average recoveries were 100.3％with RSD as 1.1％and 105.9％with RSD as 1.4％,respectively.The content results of 5 batches of Caulis Stauntoniae Chinensis tablets were 0.083-1.115 mg·g-1 for calceolarioside B and 0.161-1.204 mg·g-1 for chlorogenic acid.Conclusion:The method can be used for improving the quali-ty evaluation standard of Caulis Stauntoniae Chinensis tablets.
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