HPLC法同时测定野木瓜片中木通苯乙醇苷B和绿原酸的含量
Simultaneous determination of calceolarioside B and chlorogenic acid in Caulis Stauntoniae Chinensis tablets by HPLC
何积芬 1黄国健 2符滇海 2谢炯 2吕冠欣1
作者信息
- 1. 国家药品监督管理局药物代谢研究与评价重点实验室,广州 510515;佛山市顺德区药品检验所,广东佛山 528300
- 2. 佛山市顺德区药品检验所,广东佛山 528300
- 折叠
摘要
目的:建立同时测定野木瓜片中木通苯乙醇苷B和绿原酸含量的高效液相色谱法.方法:采用Ther-mo BDS HYPERSIL C18 色谱柱(4.6 mm × 250 mm,5 μm),柱温为35℃;以甲醇-0.1％磷酸水溶液为流动相,梯度洗脱;流速为1.0 mL·min-1;检测波长为327 nm;进样体积为10μL.结果:木通苯乙醇苷B和绿原酸分别在0.51～20.60μg·mL-1(r=1.000)和0.52～20.63μg·mL-1(r=1.000)内呈良好线性关系;平均回收率分别为100.3％(RSD为1.1％)和105.9％(RSD为1.4％).测定5批次样品,木通苯乙醇苷B和绿原酸含量分别为0.083～1.115 mg·g-1和0.161～1.204 mg·g-1.结论:本法可为野木瓜片的质量评价标准提高提供参考依据.
Abstract
Objective:To establish a method for simultaneous determination of calceolarioside B and chlorogenic acid in Caulis Stauntoniae Chinensis tablets by HPLC.Methods:The analysis was performed on a Thermo BDS HYPERSIL C18 column(4.6 mm ×250 mm,5 μm)maintained at 35 ℃.The mobile phase was consisted of meth-anol and 0.1％phosphoric acid solution,and gradient eluted with a flow rate of 1.0 mL·min-1.The detection wavelength was 327 nm,and the injection volume was 10 μL.Results:The linear ranges of calceolarioside B and chlorogenic acid were 0.51-20.60 μg·mL-1(r=1.000)and 0.52-20.63 μg·mL-1(r=1.000),respective-ly.The average recoveries were 100.3％with RSD as 1.1％and 105.9％with RSD as 1.4％,respectively.The content results of 5 batches of Caulis Stauntoniae Chinensis tablets were 0.083-1.115 mg·g-1 for calceolarioside B and 0.161-1.204 mg·g-1 for chlorogenic acid.Conclusion:The method can be used for improving the quali-ty evaluation standard of Caulis Stauntoniae Chinensis tablets.
关键词
野木瓜片/高效液相色谱法/木通苯乙醇苷B/绿原酸/含量测定Key words
Caulis Stauntoniae Chinensis tablets/HPLC/calceolarioside B/chlorogenic acid/content determination引用本文复制引用
出版年
2024
NETL
NSTL
万方数据