Simultaneous determination of four active ingredients including gentiopicrin in Jindan tablets by HPLC
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目的:建立高效液相色谱法同时测定金胆片中4种主要活性成分含量的方法.方法:采用Agilent E-clipse X DB C18(4.6 mm ×250 mm,5 μm)色谱柱;以乙腈(A)-0.1％磷酸溶液(B)为流动相梯度洗脱,检测波长为270 nm;柱温为30 ℃;流速1.0 mL·min-1.结果:龙胆苦苷、虎杖苷、槲皮素和大黄素质量浓度分别在 7.875～78.75 μg·mL-1(r=0.999 9)、6.75～67.50 μg·mL-1(r=0.999 7)、7.726～77.26 μg·mL-1(r=0.999 4)、3.809～38.09 μg·mL-1(r=0.999 8)范围内与峰面积呈良好的线性关系,平均加样回收率分别为 99.31％、99.21％、99.04％、99.59％(n=6),RSD 分别为 1.86％、1.24％、1.37％、1.15％.结论:该实验方法准确性可靠、重复性和稳定性良好,专属性强,可为金胆片的质量控制和标准提升提供参考依据.
Objective:To establish a method to determine 4 active ingredients in Jindan tablets by HPLC.Methods:Agilent Eclipse X DB C18(4.6 mm ×250 mm,5 μm)column was adoped using acetonitrile(phase A)-0.1％phophoric acid solution(phase B)as the mobile phase with gradient program at the flow rate of 1.0 mL· min-1,the detection wavelength was 270 nm,Column temperature was set at 30 ℃.Results:The linear ranges of gentiopicrin,polydatin,quercetin and emodin were 7.875-78.75 μg·mL-1(r=0.999 9),6.75-67.50 μg· mL-1(r=0.999 7),7.726-77.26 μg·mL-1(r=0.999 4),3.809-38.09 μg·mL-1(r=0.999 8),respec-tively,the peak area had a good linear relationship with the mass concentration of 4 components,the average recov-ery were 99.31％,99.21％,99.04％,99.59％,(n=6)respectively,and RSDs were 1.86％,1.24％,1.37％,1.15％,respectively.Conclusion:This method is reliable,repeatable and stable,and has strong speci-ficity.This method can provide a reference for the quality control and standard improvement of Jindan tablets.
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