Determination of astragaloside Ⅳ in Jianer Xiaoshi oral liquid by HPLC-CAD
Objective:To establish an HPLC-CAD method to determine the content of astragaloside in Jianer Xiaoshi oral liquid in order to improve the content determination method in the current standard.Methods:Agilent ZORBAX SB-C8 column(4.6 mm ×250 mm,5 μm)was adopted with acetonitrile-water(35∶65)as the mobile phaseat the flow rate of 1 mL·min-1 by gradient elution.Column temperature was room temperature,and theoretical plate number was ≥4 000.Detection parameters of electrospray detector:control mode remote(N2000,1∶local0),atomizing tube temperature 25 ℃,carrier gas(nitrogen)was at flow rate:4 L·min-1.The feasibility and relia-bility of this method for the determination of astragaloside were confirmed by various methods.Results:The method showed a good linear relationship in the concentration range of 2.25-101.68 μg·mL-1.RSD of content was 1.35％in repeatability test with average content of 0.824 mg·mL-1:The average recovery rate(n=3)was 99.2％with RSD 1.2％,which met the requirements.Conclusion:The method verified by methodology can be applied quality control of Jianer Xiaoshi oral liquid method.
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