HPLC-DAD波长切换法测定阿司匹林双嘧达莫片的含量及游离水杨酸

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中文摘要：目的:建立HPLC-DAD波长切换法测定阿司匹林双嘧达莫片的含量及游离水杨酸的方法。方法:采用 Agilent ZORBAX SB C18 色谱柱(250 mm ×4。6 mm，5 µm)，以乙腈-四氢呋喃-冰醋酸-水(20∶5∶5∶70)为流动相，流速1。0 mL·min-1，柱温30℃，检测波长276 nm(阿司匹林)、284 nm(双嘧达莫)和303 nm(水杨酸)。结果:阿司匹林、双嘧达莫和游离水杨酸分别在38。37～383。71、9。60～96。05和1。02～10。21 μg·mL-1范围内线性关系良好。平均回收率分别为99。88％(RSD=0。23％)、99。01％(RSD=0。46％)和100。99％(RSD=0。75％)。本法与标准方法含量测定结果无明显差异。含量均匀度A+2。2S在4。7～6。4。结论:建立的含量测定方法，操作简单，更适用于阿司匹林双嘧达莫片的质量控制。

外文标题：Determination of two components and salicylic acid in aspirin and dipyridamole tablets by HPLC-DAD wavelength switching method

外文摘要：Objective:To establish an HPLC-DAD wavelength-switching method for the determination of aspirin dipyridamole tablets and free salicylic acid.Methods:An Agilent ZORBAX SB C18 column(250 mm ×4.6 mm,5 μm)was adopted with acetonitrile-tetrahydrofuran-glacial acetic acid-water(20∶5∶5∶70)as the mobile phase,and isocratic elution was performed at the flow rate of 1.0 mL·min-1,the column temperature of 30 ℃,and the detection wavelengths were 276 nm for aspirin,284 nm for dipyridamole and 303 nm for salicylic acid.Results:Aspirin,dipyridamole and free salicylic acid had a good linear relationship in the range of 38.37-383.71,9.60-96.05 and 1.02-10.21 µg·mL-1 respectively.The average recoveries were 99.88％(RSD=0.23％),99.01％(RSD=0.46％)and 100.99％(RSD=0.75％),respectively.There was no signif-icant difference for the content resuls between the established method and the standard method.The content uni-formity of A+2.2S ranges from 4.7 to 6.4.Conclusion:The established method is simple and suitable for the quality control of aspirin dipyridamole tablets.

外文关键词：

aspirindipyridamolefree salicylic acidcontent determinationcontent uniformityHPLC-DAD wavelength switching method

作者：

田海燕、刘向国

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作者单位：

德州市食品药品检验检测中心,山东德州 253015

关键词：

阿司匹林双嘧达莫游离水杨酸含量测定含量均匀度 HPLC-DAD波长切换法

出版年：

2024

DOI：