Objective:To establish a UPLC method for the simultaneous determination of neochlorogenic acid,chlorogenic acid,caffeic acid,cryptochlorogenic acid,ferulic acid,scutellarin,salvianolic acid B,and apigenin in Zilongjin tablets.Methods:Waters Acquity UPLC HSS T3 column(2.1 mm × 100 mm,1.8 µm)was used.Gradient elution was performed with acetonitrile and 0.1％phosphoric acid.The injection volume was 1 µL,the column temperature was set at 30 ℃ the flow rate was 0.3 mL·min-1,and detection was carried out at 323 nm.Results:The linear relationship between the 8 components within their respective mass concentration ranges was good(r＞0.999 0),with average recovery of 94.1％to 101.6％and RSD％of 0.70％to 1.93％.Four batches of sam-ples were tested,and the content of the other components in different batches was basically same,except slight differ-ences in the content of neochlorogenic acid,chlorogenic acid,and cryptochlorogenic acid.Conclusion:This method is simple,efficient,and accurate,and can comprehensively evaluate the intrinsic quality of this product,which pro-vides a better reference for the quality evaluation of Zilongjin tablets.
维普
万方数据
