Speciation analysis of mercury in Huoxue Zhitong capsules by HPLC-HG-AFS
Objective:To determine divalent mercury,methylmercury and ethylmercury in Huoxue Zhitong capsules,and to provide data support for the risk assessment and management of traditional medicines products.Methods:High performance liquid chromatography-hydrogenation-atomic fluorescence spectrometry(HPLC-HG-AFS)was used to determine the content of three forms of mercury(divalent mercury,Hg2+;methyl mercury,CH3 Hg;ethyl mercury,C2H5Hg)in different batches of Huoxue Zhitong capsules.Chromatographic conditions were C18 column(250 mm ×4.6 mm,5 μ·m)and acetonitrile-0.06 mol·L-1 ammonium acetate solution(containing 0.01 mol·L-1 L-cysteine)(5∶95)as a mobile phase.Hydride generation parameters were oxidant agent of 1％potassium persulfate-0.35％potassium hydroxide mixed solution,reducing agent of 2.0％potassium borohydride-0.35％potassium hydroxide mixed solution,carrier solution of 7％hydrochloric acid,negative high pressure of 285 V,and atomizer height of 10 mm.Results:The linear range of mercury species was 1.00-10.0 μg·L-1 with r＞0.999.The detection limit values were 0.088-0.131 ng·L-1,and the average recoveries ranged from 91.98％to 93.10％with RSD of＜5.73％(n=3).Three mercury forms in different batches of Huoxue Zhitong capsules by HPLC-HG-AFS were below the detection limits.Conclusion:The validated method can be used for the determination of mercury speciation in Huoxue Zhitong capsules and other Chinese patent medicines,which pro-vides a reference for the safety assessment of Chinese patent medicines.
万方数据
维普
