HPLC法测定赖氨酸维B12颗粒中香草醛的含量

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中文摘要：目的:建立HPLC法测定赖氨酸维B12颗粒中香草醛含量的方法。方法:取赖氨酸维B12颗粒1。0 g，置10 mL刻度离心管中，加水1 mL溶解后，涡旋1 min，加甲醇至刻度后，继续涡旋1 min进行提取。采用Agi-lent C18(250 mm ×4。6 mm，5 μm)色谱柱，流动相为甲醇-水(35∶65)，检测波长为277 nm。结果:本方法香草醛检出限为0。2 μg·g-1，浓度在1。962～49。04μg·mL-1的范围内呈线性关系(r=0。999 9)，低、中、高三水平回收率在91。86％～101。61％之间，RSD为2。9％(n=9)。测定样品6批，A企业4批，香草醛含量在55。18～79。55μg。g-1，B企业和C企业均未检出。结论:该方法操作简便，结果可靠，可用于赖氨酸维B12颗粒中香草醛含量的测定。

外文标题：Determination of vanillin in lysine and vitamin B12 granules by HPLC

外文摘要：Objective:To establish an HPLC method to determine of vanillin in lysine and vitamin B12 granules.Methods:1.0 g of lysine and vitamin B12 granules was put into a 10mL graduated centrifuge tube,1 mL of water was added to dissolve,vortex for 1 min,and methanol was added to the scale and vortex for 1 min for extraction.The samples were separated on a C18 column(250 mm × 4.6 mm,5 μm)with a mobile phase of methanol and water(35∶65)at ta flow rate of 1.0 mL·min-1.The detection wavelength was 277 nm.Results:The limit of detection of vanillin was 0.2 µg·g-1,and the concentration was linear in the range of 1.962-49.04 µg·mL-1(r=0.999 9).The recoveries of vanillin at low,medium and high levels were 91.86％-101.61％with RSD of 2.9％(n=9).6 batches of samples were determined,and the results showed that the vanillin content in 4 batches of enterprise A was 55.18-79.55 μg·g-1,and no vanillin was detected in enterprise B and enterprise C.Conclusion:The method is simple and reliable,and can be used for the rapid determination of vanillin in lysine and vitamin B12 granules.

外文关键词：

lysine and vitamin B12 granulesvanillinHPLC methodpharmaceutical excipientflavoring agent

作者：

田海燕、刘娟、刘维彬、刘洪海

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作者单位：

德州市食品药品检验检测中心,山东德州 253000

关键词：

赖氨酸维B12颗粒香草醛含量 HPLC法辅料矫味剂

出版年：

2024

DOI：