Quality evaluation of zhibitai capsules based on high performance liquid chromatography QAMS combined with chemometrics
Objective:To establish a method for the quality evaluation of zhibitai capsules by high performance liquid chromatography-quantitative analysis of multi-components by single marker(HPLC-QAMS)combined with chemometrics.Methods:HPLC method was used to establish the relative correction factors(f)of alisol A with vitexin-4"-O-glucoside,rhamnosylvitexin,oleanolic acid,ursolic acid,atractylenolide Ⅲ,atracylenolide Ⅰ,alisol A 24-acetate and 23-acetate alisol B by external standard method with alisol A as the internal reference.The estab-lished f was used to calculate the contents of the corresponding eight components,and the differences between the measured values and the calculated values were compared.The content data were analyzed by chemometrics to evaluate its quality.Results:The f values of alisol A with vitexin-4"-O-glucoside,rhamnosylvitexin,oleanolic acid,ursolic acid,atractylenolide Ⅲ,atracylenolide Ⅰ,alisol A 24-acetate and 23-acetate alisol B were 1.044 0,0.633 1,0.861 0,0.691 7,1.224 8,1.372 7,1.516 1 and 0.768 2,respectively,and the repeatability was good.Nine components in 13 batches of zhibitai capsules were determined by QAMS method and external standard method,and the results were not significantly different.The results of principal component analysis showed that 13 batches of samples were clustered into 3 categories.The main quality difference factors of zhibitai capsule were rhamnosylvitexin,ursolic acid,atractylenolide Ⅲ,atracylenolide Ⅰ and 23-acetyl alisol B.Conclusion:The estab-lished method can be used for the comprehensive evaluation of the quality of zhibitai capsules.
zhibitai capsulesHPLC-QAMSchemometricsstatistics softwareprincipal component analysispartial least squares discrimination analysis
万方数据
维普
