摘要
目的:建立高效液相色谱法测定肤痒颗粒中绿原酸、阿魏酸和羟基红花黄色素A含量的方法.方法:采用资生堂CAPCELL PAK C18 MGⅡ(4.6 mm ×250 mm,5 μm)色谱柱,甲醇-0.05％的磷酸水溶液(30∶70)为流动相,检测波长为322 nm(绿原酸、阿魏酸);403 nm(轻基红花黄色素A);流速为1 mL·min-1;柱温为30 ℃.结果:绿原酸、阿魏酸和羟基红花黄色素A的线性范围分别为105.9～1 059 ng,r=0.999 9;74.04～740.4 ng,r=0.999 9;14.97～149.7 ng,r=0.999 9.平均回收率分别为 98.74％、96.12％和103.9％,RSD分别为0.96％、1.83％和1.34％.测定35批样品,结果含糖颗粒每袋中含有绿原酸、阿魏酸和羟基红花黄色素A的量分别在0.56～4.49 mg、0.13～1.65 mg和0.36～2.60 mg之间.无糖颗粒每袋中含有绿原酸、阿魏酸和羟基红花黄色A的量分别在2.47～2.98 mg、0.31～0.33 mg和0.49～0.56 mg之间.结论:该方法操作简便、重复性好、结果准确,可作为肤痒颗粒的控制方法.
Abstract
Objective:Establish a method for determining the content of chlorogenic acid,ferulic acid,and hydroxysafflower yellow A in fuyang granules by high-performance liquid chromatography.Methods:Adopting Shiseido CAPCELL PAK C18 MG Ⅱ(4.6 mm × 250 mm,5 μm)chromatographic column,Methanol-0.05％phosphoric acid aqueous solution(30:70)was used as the mobile phase,the detection wavelength was 322 nm(chlorogenic acid,ferulic acid);403 nm(hydroxysafflower yellow A);the flow rate was 1 mL·min-1;the column temperature was 30 ℃.Results:The linear ranges of chlorogenic acid,ferulic acid,and hydroxysafflower yellow A were 105.9 to 1 059 ng,r=0.999 9,respectively;74.04-740.4 ng,r=0.999 7;14.97-149.7 ng,r=0.999 8.The average recovery were 98.74％,96.12％,and 103.9％,respectively,with RSDs of 0.96％,1.83％,and 1.34％.35 batches of samples were tested,and the results showed that each bag of sugar containing particles contained amounts of chlorogenic acid,ferulic acid,and hydroxysafflower yellow A,ranging from 0.56 to 4.49 mg,0.13 to 1.65 mg,and 0.36 to 2.60 mg,respectively.The amount of chlorogenic acid,ferulic acid,and hydroxysafflower yellow A contained in each bag of sugar free particles ranged from 2.47 to 2.98 mg,0.31 to 0.33 mg,and 0.49 to 0.56 mg,respectively.Conclusion:This method is easy to operate,has good repeatability,and accurate results,and can be used as a control method for fuyang granules.
维普
万方数据