Objective:Establish a method for determining the content of chlorogenic acid,ferulic acid,and hydroxysafflower yellow A in fuyang granules by high-performance liquid chromatography.Methods:Adopting Shiseido CAPCELL PAK C18 MG Ⅱ(4.6 mm × 250 mm,5 μm)chromatographic column,Methanol-0.05％phosphoric acid aqueous solution(30:70)was used as the mobile phase,the detection wavelength was 322 nm(chlorogenic acid,ferulic acid);403 nm(hydroxysafflower yellow A);the flow rate was 1 mL·min-1;the column temperature was 30 ℃.Results:The linear ranges of chlorogenic acid,ferulic acid,and hydroxysafflower yellow A were 105.9 to 1 059 ng,r=0.999 9,respectively;74.04-740.4 ng,r=0.999 7;14.97-149.7 ng,r=0.999 8.The average recovery were 98.74％,96.12％,and 103.9％,respectively,with RSDs of 0.96％,1.83％,and 1.34％.35 batches of samples were tested,and the results showed that each bag of sugar containing particles contained amounts of chlorogenic acid,ferulic acid,and hydroxysafflower yellow A,ranging from 0.56 to 4.49 mg,0.13 to 1.65 mg,and 0.36 to 2.60 mg,respectively.The amount of chlorogenic acid,ferulic acid,and hydroxysafflower yellow A contained in each bag of sugar free particles ranged from 2.47 to 2.98 mg,0.31 to 0.33 mg,and 0.49 to 0.56 mg,respectively.Conclusion:This method is easy to operate,has good repeatability,and accurate results,and can be used as a control method for fuyang granules.
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