Rapid determination of total content of bufalin,cinobufotalin and lipobufotalin in Bufonis Venenum pulp by solid phase extraction(SPE)-UV spectrometry
Objective:To establish a method for SPE purification of Bufonis Venenum pulp and determination of total content of bufalin,cinobufotalin and lipobufotalin in Bufonis Venenum pulp by UV spectrophotometry.Methods:Using Waters Oasis HLB solid-phase extraction small column to purify Bufonis Venenum pulp,the cleaning solution was 29％acetonitrile,the cleaning volume was 5 mL,the eluent was 95％acetonitrile,the elution volume was 6 mL,the elution was detected by UV spectrophotometer and the detection wavelength was 295 nm.Results:The method has good linear relationship(r＞0.999),high precision(RSD＜5％),good repeatability(RSD＜5％),and the sample recovery rate within in the ranges of 96.34％-104.76％,which meets the requirements.Conclusion:The sample processing method was rapid and simple,with good repeatabili-ty,high accuracy and high precision.As the quality control of Bufonis Venenum medicinal material intermediate,it fills the gap in the detection method of Bufonis Venenum pulp.
万方数据
维普
