首页|HPLC法测定雷公藤多苷片中雷公藤内酯甲的含量

HPLC法测定雷公藤多苷片中雷公藤内酯甲的含量

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  • NETL
  • NSTL
  • 万方数据
  • 维普
目的:建立高效液相色谱法测定雷公藤多苷片中雷公藤内酯甲的含量。方法:样品以甲醇提取后直接测定，使用Phenomenex Gemini C18色谱柱(250 mm×4。6 mm，5μm)，以0。02 mol·L-磷酸二氢钾溶液-乙腈(32∶68)为流动相，流速1。0ml·min-1，检测波长为210 nm，柱温:室温。结果:雷公藤内酯甲线性范围为18。0～90。0μg·ml-1(r=0。999 7)，平均回收率为102。61％，RSD为0。83％(n=6)。结论:本方法简便、准确、重复性好，可用于雷公藤多苷片的质量控制。
Determination of Wilforlide A in Tripterygium Tablets by HPLC
Objective: To establish a method for determination of wilforlide A in tripterygium tablets by HPLC. Method: Samples were extracted with methanol,the HPLC analysis was performed nn a Phenomenex Gemini C18(250 mm ×4.6 mm,5 μm) column,the mobile phase was 0. 02 mol·L-1 monobasic potassium phosphate - acetonitrile (32-68) wilh a flow rate of 1. 0 ml·min-1,the detection wavelength was 210nm and the analysis was operated at room temperature. Result: The linear range of wilforlide A was 18. 0- 90. 0 μg ·ml-1(r-0. 9997), and the average recovery was 102.61% with RSD of 0. 83% (n= 6). Conclusion: The method is convenient, accurate and suitable for the quality control of tripterygium tahlets.

Tripterygium tabletsWilforlide AHPLC

农凤鸣、陆兴毅

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百色食品药品检验所 广西百色533000

雷公藤多苷片 雷公藤内酯甲 高效液相色谱法

2011

中国药师
国家药品监督管理局高级研修学院,武汉医药（集团）股份有限公司

中国药师

CSTPCD
影响因子：0.944
ISSN：1008-049X
年,卷(期)：2011.14(4)
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