HPLC Fingerprints of Flavonoid in Radix Astragali from Various Habitats
Objective: To establish an HPLC fingerprints analysis method for flavonoid in Radix Astragali from various habitats. Method: The flavonoid was determined by HPLC. The mobile phase consisted of acetonitrile and water at a flow rate of 0. 8 ml·min-1 with gradient elution. The UV detection wavelength was set at 260 nm and the column temperature was 35℃. Result: There were 16 common peaks in the chromatogram of each sample. The profile of HPLC fingerprints and the ratios of the common peak area for 10 samples were approximately the same. The sum of the common peak area was more than 90% of the total peak area of each sample, while there were some differences in the ratios of the common peak area among the samples. Conclusion: The method shows promising repeatability and reproducibility. The fingerprints can be used as a quality control method for Radix Astragali.
NETL
NSTL
万方数据
维普
