首页|HPLC法同时测定田基黄颗粒中3种黄酮类成分的含量

HPLC法同时测定田基黄颗粒中3种黄酮类成分的含量

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目的:建立测定田基黄颗粒中3种黄酮类成分含量的高效液相色谱方法。方法:用ZORBAX Eclipse XDB-C18色谱柱,流动相为甲醇-0.2%磷酸溶液(42∶58),检测波长为350 nm,流速1.0 ml· min-1。结果:异槲皮苷、槲皮苷、槲皮素分别在4.572~114.300μg · ml-1、7.028~175.700μg · ml-1、2.195~54.880μg · ml-1浓度范围内呈良好线性关系( r 分别为0.9998、0.9998、0.9999);平均加样回收率分别为98.01%( RSD=1.24%,n=6)、98.14%( RSD=0.98%,n=6)、97.00%(RSD=1.09%,n=6)。结论:该方法专属性强、操作简便,可以用于田基黄颗粒的质量控制。
Simultaneous Determination of Three Flavonoids in Tianjihuang Granules by HPLC
Objective: To establish an HPLC method for the simultaneous determination of three flavonoids in Tianjihuang gran -ules.Methods:A ZORBAX Eclipse XDB-C18 column was used.The mobile phase was methanol-0.2%phosphoric acid solution (42∶58).The detection wavelength was 350 nm and the flow rate was 1.0 ml· min-1.Results:The linear range of isoquercitrin, quer-citrin and quercetin was 4.572-114.300 μg· ml-1(r=0.999 8), 7.028-175.700 μg· ml-1(r=0.999 8) and 2.195-54.880 μg· ml-1(r=0.999 9), respectively.The average recovery was 98.01%(RSD=1.24%, n=6), 98.14%(RSD=0.98%, n=6) and 97.00%(RSD=1.09%, n=6) , respectively.Conclusion:The method is specific and simple,which can be used for the quality con-trol of Tianjihuang granules.

HPLCTianjihuang granulesIsoquercitrinQuercitrinQuercetin

赵丹、邓祖磊、葛德洲、刘浩

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蚌埠医学院药学系 安徽蚌埠233000

蚌埠市食品药品检验中心

高效液相色谱法 田基黄颗粒 异槲皮苷 槲皮苷 槲皮素

2016

中国药师
国家药品监督管理局高级研修学院,武汉医药(集团)股份有限公司

中国药师

CSTPCD
影响因子:0.944
ISSN:1008-049X
年,卷(期):2016.19(7)
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