Objective To quickly analyze and identify the chemical constituents in Zhuangxi-6 powders by HPLC-Q-Exactive-MS.Methods The separation was performed on Shim-pack GIST C18 column(150 mm×4.6 mm,5 μm)with the mobile phase of methanol-0.1%formic acid aqueous solution(gradient elution).The flow rate was 0.35 mL/min,column temperature was 35℃,and the volume of sample injection was 10 μL.Electrospray ionization was applied for scanning under positive and negative ion modes with the scanning range of m/z 110-1 200.Results Combining mass spectrometry data and literature information,a total of 65 components were identified in Zhuangxi-6 powders,including 16 alkaloids,11 flavonoids,14 organic acids,6 chromones,7 sesquiterpenoids,4 fatty acids,3 amino acids,2 tannins,1 phenolic acids,and 1 phenylpropanoid.Conclusion HPLC-Q-Exactive-MS method can quickly identify the chemical components of Zhuangxi-6 powders,laying a foundation for the further research on the pharmacodynamics substances of Zhuangxi-6 powders.
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