首页|樟帮法甘草制吴茱萸指纹图谱的建立及生物碱类成分的含量测定

樟帮法甘草制吴茱萸指纹图谱的建立及生物碱类成分的含量测定

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目的 建立樟帮法甘草制吴茱萸(以下简称"泡吴茱萸")的HPLC指纹图谱，并同时测定吴茱萸碱、吴茱萸次碱、吴茱萸新碱的含量，为泡吴茱萸的质量控制及标准提高提供依据。方法 基于多波长切换法建立泡吴茱萸的指纹图谱，并进行相似度评价;以吴茱萸次碱为内参物，采用斜率校正法计算吴茱萸碱、吴茱萸新碱的相对校正因子，并比较一测多评(QAMS)法与外标法测定结果的差异。结果 建立了泡吴茱萸的指纹图谱，共有 20 个共有峰，与对照指纹图谱的相似度为 0。970～0。998，共指认了 7 个共有峰;10 批样品中QAMS法测定的吴茱萸碱、吴茱萸新碱的含量为1。754～7。542 mg/g和 1。281～2。455 mg/g，QAMS法与外标法两种检测方法所得结果相似。结论 所建立的HPLC法简单、可靠，分离度、重复性良好，可用于泡吴茱萸的质量评价。
Establishment of the fingerprints of soaking Euodiae fructus with water decoction of Radix glycyrrhizae by Zhangbang method and content determination of alkaloid
Objective To establish HPLC fingerprints of soaking Euodiae fructus with water decoction of Radix glycyrrhizae by Zhangbang method(hereinafter referred to as"soaking Euodiae fructus"),and to determine the content of evodiamine,rutaecarpine and evocarpine,so as to provide the basis for the quality control and standard improvement of soaking Euodiae fructus.Methods The fingerprint of soaking Euodiae fructus was established based on wavelength switching technology,and the similarity evaluation was conducted.Taking the rutaecarpine as the internal reference,the relative correction factors of evodiamine and evocarpine were calculated by slope correction method,and the differences in measurement results between quantitative analysis of multi-components by single-marker(QAMS)method and external standard method were compared.Results The established fingerprint of soaking Euodiae fructus had a total of 20 common peaks,the similarity between it and the control fingerprint spectrum was 0.970 to 0.998,and 7 common peaks of them were identified.The contents of evodiamine and evocarpine determined by QAMS method were 1.754-7.542 mg/g and 1.281-2.455 mg/g in 10 batches of samples,and the results obtained by QAMS method and external standard method were similar.Conclusion The established HPLC method is simple,reliable,with good separation and repeatability,and can be used for quality evaluation of processed Euodiae fructus.

Soaking Euodiae fructus with water decoction of Radix glycyrrhizaeZhangbang methodProcessing technologyHigh performance liquid chromatographyFingerprintAlkaloidQuantitative analysis of multi-components by single-markerContent determination

姜军华、陈建兰、许妍、刘绪平

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江西省药品检验检测研究院(南昌 330029)

国家药品监督管理局中成药质量评价重点实验室(南昌 330029)

江西省药品与医疗器械质量工程技术研究中心(南昌 330029)

南昌县中医院药剂科(南昌 330200)

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甘草制吴茱萸 樟帮法 炮制工艺 高效液相色谱法 指纹图谱 生物碱 一测多评法 含量测定

江西省中医药标准化研究项目

2021A12

2024

10.12173/j.issn.1008-049X.202401116

中国药师
国家药品监督管理局高级研修学院,武汉医药（集团）股份有限公司

中国药师

CSTPCD
影响因子：0.944
ISSN：1008-049X
年,卷(期)：2024.27(2)
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