Quality difference evaluation of Radix tetrastigmae based on quantitative analysis of multi-components by single-marker,chemometrics and entropy weight-technique for order preference by similarity to ideal solution method
Objective To evaluate the quality difference of Radix tetrastigmae from different producing areas by quantitative analysis of multi-components by single-marker(QAMS),chemometrics and entropy weight-technique for order preference by similarity to ideal solution(EW-TOPSIS)method,and to provide basis for studying the geoherbalism of Radix tetrastigmae.Methods 18 batches of Radix tetrastigmae samples were collected from 6 provinces including Chongqing,Guangxi,Hunan,Guangdong,Zhejiang and Jiangsu.The contents of chlorogenic acid,caffeic acid,catechin,procyanidin B1,orientin,rutoside,polydatin,isoquercitrin,kaempferol-3-O-rutinoside,astragalin,resveratrol and kaempfero in Radix tetrastigmae were simultaneously determined by HPLC-QAMS,and a multi-component quantitative control mode for Radix tetrastigmae was established.The main potential markers affecting the quality of Radix tetrastigmae were screened by chemical pattern recognition such as principal component analysis and orthogonal partial least squares-discriminant analysis.The EW-TOPSIS was used to establish the quality evaluation model of Radix tetrastigmae,and the quality difference was comprehensively evaluated.Results The 12 components showed good linear relationships in their own ranges.The average recovery were 96.89%-100.13%,with the RSDs﹤2.0%(n=9).There was no significant difference between the calculated values of HPLC-QAMS and the measured values of external standard method.The cumulative variance contribution rate of the first two principal components was 90.15%.The proanthocyanidin B1,catechin,rutoside,orientin and chlorogenic acid may be the main potential markers affecting the quality of Radix tetrastigmae.The results of EW-TOPSIS analysis showed that the relative correlation of 18 batches of Radix tetrastigmae were 0.154 9-0.732 0.Conclusion The established method is convenient in operation and accurate in results,and can be used as a quantitative control method for multi-components of Radix tetrastigmae.The chemometrics and EW-TOPSIS method are objective and comprehensive,and can be used for the overall quality difference evaluation of Radix tetrastigmae.
Radix tetrastigmaeQuantitative analysis of multi-components by single-markerChemometricsEntropy weight-technique for order preference by similarity to ideal solutionQuality difference evaluation
万方数据
维普
