Objective To establish a method for the determination of related substances in glutaric anhydride by HPLC.Methods The HPLC method was performed on a Waters Symmetry C18 column(250 mm×4.6 mm,5 µm)with mobile phase of methanol-0.1%phosphoric acid solution(3 ∶ 97)at a flow rate of 0.9 mL/min,the column temperature was 30℃,the injection volume was 20 μL,and the detection wavelength was set at 210 nm.Results Succinic acid,adipic acid and unknown impurities in glutaric anhydride can be effectively detected.The linear range was 5.925-59.251 μg/mL for succinic acid(r=0.999 6),6.210-62.100 μg/mL for adipic acid(r=0.999 7),and 5.110-8 516.000 μg/mL for glutaric acid(r=0.999 9).The average recoveries of succinic acid and adipic acid were 100.92%(RSD=0.86%)and 105.89%(RSD=2.99%)(n=9),respectively.Conclusion The method has strong specificity,high sensitivity and good accuracy,and can be used for the related substances determination of glutaric anhydride.
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