首页|桦褐孔菌的生药学鉴别研究

桦褐孔菌的生药学鉴别研究

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目的 研究中药材桦褐孔菌的生药鉴别方法及特征。方法 从东北原产地采集样品，采用来源、分子生物学、性状、显微等鉴定法和HPLC法对桦褐孔菌药材和其非纯粹菌体、伪品进行研究。结果 桦褐孔菌外形为瘤状团块，微观构造为菌丝紧密交联状，药用部位为菌核，而非当前中药材标准所述的"子实体"。桦褐孔菌药材中偶见极少量的菌管组织与菌肉。管孔类圆形，直径 130～190 μm，破裂;管壁构造特别，由纵向排列的直径为 2～5～8 μm的小包室构成。非纯粹菌体系被菌丝渗透已腐朽的宿主木质部，所含栓菌酸和麦角甾醇含量显著低于纯粹菌核(P＜0。05)。桦褐孔菌与伪品树瘤的显微结构和HPLC指纹图谱区别明显。结论 所用方法能对桦褐孔菌药材进行真伪鉴别以及药材品质辨识。
Pharmacognostical identification research of Inonotus obliquus
Objective To study the identification methods and traits of the Chinese medicinal materials Inonotus obliquus.Methods Samples were collected from the origin of northeast China,and source identification,molecular biology identification,trait identification,microscopic identification and HPLC assay were used to study the Inonotus obliquus,its non-pure fungous part,and counterfeit.Results Inonotus obliquus had a nodular shape,the microstructure was closely interconnected with mycelia,and the medicinal part was the sclerotium,not the"fruiting body"as stipulated in the current standards of traditional Chinese medicinal materials.Occasionally,a very small amount of tubule and subiculum could be seen in Inonotus obliquus.Tubule hole shape was circular,with a diameter of 130-190 μm,and ruptured.The pipe wall structure was special,which was composed of longitudinal arranged small compartment with diameter of 2-5-8 μm.Non-pure fungous part was the decayed host xylem infiltrated by mycelia.The contents of trametenolic acid and ergosterol in this part were significantly lower than the pure sclerotium(P<0.05).The microstructure and HPLC fingerprints of Inonotus obliquus and the counterfeit burl were clearly distinguishable from each other.Conclusion The method used is able to identify the authenticity of Inonotus obliquus and the quality of the medicinal materials.

Inonotus obliquusMedicinal partsBasal source investigationTrait identificationMicroscopic identificationMolecular biologyHigh-performance liquid chromatographyContent determination

朱田密、段雨晴、王思萌、陈树和、杨莹、陈科力

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湖北省中医院/湖北中医药大学附属医院/湖北省中医药研究院药事部(武汉 430061)

湖北中医药大学药学院(武汉 430065)

中国科学院微生物研究所(北京 100101)

桦褐孔菌 药用部位 基原调查 性状鉴别 显微鉴别 分子生物学 高效液相色谱法 含量测定

湖北省自然科学基金创新发展联合基金项目第七批全国老中医药专家学术经验继承工作项目

2022CFD162国中医药人教函[2022]76号

2024

10.12173/j.issn.1008-049X.202312062

中国药师
国家药品监督管理局高级研修学院,武汉医药（集团）股份有限公司

中国药师

CSTPCD
影响因子：0.944
ISSN：1008-049X
年,卷(期)：2024.27(7)