10种中药饮片污染微生物群落特征研究
Community characteristics of contaminated microorganisms in 10 kinds of Chinese herbal pieces
蓝福胜 1吴燕燕 1林玉双 1郑域茹 1高丹玲1
作者信息
- 1. 福建省食品药品质量检验研究院(福州 350001)
- 折叠
摘要
目的 考察10种中药饮片微生物污染水平,并对其污染的真菌和耐热菌开展多样性研究.方法 根据《中国药典(2020年版)》微生物限度检查方法,检测饮片的需氧菌总数(TAMC)、霉菌和酵母菌总数(TYMC)、耐热菌总数(THRC)和3种控制菌,基于内转录间隔区(ITS)和16S核糖体DNA(16SrDNA)高通量测序技术研究饮片污染真菌和耐热菌的群落特征.结果 28％(14/50)TAMC大于106 cfu/g,20％(10/50)TYMC大于104 cfu/g,68％(34/50)THRC大于10 cfu/g,40％(20/50)检出耐胆盐革兰阴性菌,2批广藿香检出大肠埃希菌,所有饮片均未检出沙门氏菌.高通量测序结果显示饮片污染真菌分布于12门、777属、1 467种,耐热菌分布于5门、57属、74种;饮片中含有黄曲霉菌以及解淀粉芽孢杆菌、缓慢葡萄球菌和沃氏葡萄球菌等耐热性致病菌.结论 这10种中药饮片普遍受微生物污染,部分饮片含有产毒真菌和致病性耐热菌,患者用药具有潜在的微生物致病风险.
Abstract
Objective To evaluate the microbial contamination levels of 10 kinds of Chinese herbal pieces,and explore the diversity of fungi and heat-resistant bacteria.Methods The total aerobic microbial count(TAMC),total yeast and mould count(TYMC),total heat-resistant microbial count(THRC)and 3 types control bacteria were detected according to the microbial limit inspection method of Chinese Pharmacopoeia 2020.Besides,the community characteristics of contaminated fungi and heat-resistant bacteria on prepared slices were analysed based on internal transcribed spacer(ITS)and 16S ribosomal DNA(16SrDNA)high-throughput sequencing method.Results The TAMC of 28％(14/50)samples exceeded 106 cfu/g,the TYMC of 20％(10/50)samples exceeded 104 cfu/g,the THRC of 68％(34/50)samples were higher than 10 cfu/g,and the bile-tolerant gram-negitive bacteria were detected in 40％(20/50)of the samples.Escherichia coli were found in 2 batches of the Pogostemon cablin,but Salmonella was not found in any sample.High throughput sequencing results showed that the fungi contaminated distributed in 12 phylums,777 genera and 1 467 species;the heat-resistant bacteria were mainly distributed in 5 phylums,57 genera and 74 species.The decoction pieces contained Aspergillus flavus and heat-resistant pathogens such as Bacillus amyloliquefaciens,Staphylococcus lentus,and Staphylococcus warneri.Conclusion The 10 kinds of decoction pieces are generally contaminated by microorganisms,and some of them contain toxic fungi and pathogenic heat-resistant bacteria,which poses a potential risk of microbial pathogenicity for patients.
关键词
中药饮片/微生物污染/控制菌检查/真菌/耐热菌/高通量测序/菌种鉴定/黄曲霉菌/解淀粉芽孢杆菌/缓慢葡萄球菌/沃氏葡萄球菌Key words
Chinese herbal pieces/Microbial contamination/Control bacteria test/Fungi/Heat-resistant bacteria/High-throughput sequencing/Species identification/Aspergillus flavus/Bacillus amyloliquefaciens/Staphylococcus lentus/Staphylococcus warneri引用本文复制引用
出版年
2024
NETL
NSTL
万方数据