10种中药饮片污染微生物群落特征研究

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中文摘要：目的考察10种中药饮片微生物污染水平，并对其污染的真菌和耐热菌开展多样性研究。方法根据《中国药典(2020年版)》微生物限度检查方法，检测饮片的需氧菌总数(TAMC)、霉菌和酵母菌总数(TYMC)、耐热菌总数(THRC)和3种控制菌，基于内转录间隔区(ITS)和16S核糖体DNA(16SrDNA)高通量测序技术研究饮片污染真菌和耐热菌的群落特征。结果 28％(14/50)TAMC大于106 cfu/g，20％(10/50)TYMC大于104 cfu/g，68％(34/50)THRC大于10 cfu/g，40％(20/50)检出耐胆盐革兰阴性菌，2批广藿香检出大肠埃希菌，所有饮片均未检出沙门氏菌。高通量测序结果显示饮片污染真菌分布于12门、777属、1 467种，耐热菌分布于5门、57属、74种;饮片中含有黄曲霉菌以及解淀粉芽孢杆菌、缓慢葡萄球菌和沃氏葡萄球菌等耐热性致病菌。结论这10种中药饮片普遍受微生物污染，部分饮片含有产毒真菌和致病性耐热菌，患者用药具有潜在的微生物致病风险。

外文标题：Community characteristics of contaminated microorganisms in 10 kinds of Chinese herbal pieces

外文摘要：Objective To evaluate the microbial contamination levels of 10 kinds of Chinese herbal pieces,and explore the diversity of fungi and heat-resistant bacteria.Methods The total aerobic microbial count(TAMC),total yeast and mould count(TYMC),total heat-resistant microbial count(THRC)and 3 types control bacteria were detected according to the microbial limit inspection method of Chinese Pharmacopoeia 2020.Besides,the community characteristics of contaminated fungi and heat-resistant bacteria on prepared slices were analysed based on internal transcribed spacer(ITS)and 16S ribosomal DNA(16SrDNA)high-throughput sequencing method.Results The TAMC of 28％(14/50)samples exceeded 106 cfu/g,the TYMC of 20％(10/50)samples exceeded 104 cfu/g,the THRC of 68％(34/50)samples were higher than 10 cfu/g,and the bile-tolerant gram-negitive bacteria were detected in 40％(20/50)of the samples.Escherichia coli were found in 2 batches of the Pogostemon cablin,but Salmonella was not found in any sample.High throughput sequencing results showed that the fungi contaminated distributed in 12 phylums,777 genera and 1 467 species;the heat-resistant bacteria were mainly distributed in 5 phylums,57 genera and 74 species.The decoction pieces contained Aspergillus flavus and heat-resistant pathogens such as Bacillus amyloliquefaciens,Staphylococcus lentus,and Staphylococcus warneri.Conclusion The 10 kinds of decoction pieces are generally contaminated by microorganisms,and some of them contain toxic fungi and pathogenic heat-resistant bacteria,which poses a potential risk of microbial pathogenicity for patients.

外文关键词：

Chinese herbal piecesMicrobial contaminationControl bacteria testFungiHeat-resistant bacteriaHigh-throughput sequencingSpecies identificationAspergillus flavusBacillus amyloliquefaciensStaphylococcus lentusStaphylococcus warneri

作者：

蓝福胜、吴燕燕、林玉双、郑域茹、高丹玲

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作者单位：

福建省食品药品质量检验研究院(福州 350001)

关键词：

中药饮片微生物污染控制菌检查真菌耐热菌高通量测序菌种鉴定黄曲霉菌解淀粉芽孢杆菌缓慢葡萄球菌沃氏葡萄球菌

出版年：

2024

DOI：

10.12173/j.issn.2097-4922.202404114

中国药师

国家药品监督管理局高级研修学院,武汉医药（集团）股份有限公司

中国药师

CSTPCD

影响因子：0.944

ISSN：1008-049X

年,卷(期)：2024.28(11)