超滤离心-HPLC法测定鲁索替尼固体脂质纳米粒的包封率
Determination of encapsulation rate of ruxolitinib solid lipid nanoparticles by ultrafiltration centrifugation-HPLC method
冯世权 1于佳岐 2董德峤 1甘昌冉 1彭浩洋 1段成成 1秦贞苗 1高亚男1
作者信息
- 1. 海南医科大学药学院,海口 571199
- 2. 海南医科大学国际教育学院,海口 571199
- 折叠
摘要
目的 建立鲁索替尼固体脂质纳米粒(Ru-SLN)包封率的测定方法.方法 以熔融乳化-超声法制备Ru-SLN,以超滤离心法分离游离药物和Ru-SLN,采用HPLC法测定Ru的含量,并计算其包封率.结果 Ru在4.08～408.00μg/mL浓度范围内线性关系良好(r=0.9997),平均回收率为101.60％,RSD为1.09％(n=9);超滤离心法选择截留分子量为10 kDa的超滤离心管,10961×g离心10 min,无显著膜吸附作用,测得Ru-SLN的包封率为(97.01±1.23)％.结论 超滤离心法结合HPLC法测定Ru-SLN包封率快速便捷、准确性高,可为Ru制剂含量及包封率的测定提供有效的试验指导.
Abstract
Objective To establish a method for determining the encapsulation rate of ruxolitinib solid lipid nanoparticles (Ru-SLN). Methods Ru-SLN was prepared using the melt emulsification-ultrasonic method,and the free drug and Ru-SLN were separated using ultrafiltration centrifugation. The Ru content was determined using HPLC,and the encapsulation rate was calculated accordingly. Results Ru had a good linear relationship within the concentration range of 4.08-408.00 μg/mL (r=0.9997),and its average recovery rate was 101.60％,with the RSD of 1.09％ (n=9). The ultrafiltration centrifugation selected ultrafiltration centrifuge tubes with a cut-off molecular weight of 10 kDa,centrifuged at 10961 × g for 10 minutes,and showed no significant membrane adsorption. The encapsulation rate of Ru-SLN was determined to be (97.01±1.23)％. Conclusion The combination of ultrafiltration centrifugation and HPLC method for determining the encapsulation efficiency of Ru-SLN is fast,convenient,and highly accurate,providing effective experimental guidance for the determination of Ru formulation content and encapsulation effciency.
关键词
鲁索替尼/固体脂质纳米粒/熔融乳化超声法/高效液相色谱法/超滤离心法/包封率/方法学Key words
Ruxolutinib/Solid lipid nanoparticles/Melt-emulsification ultrasonic method/High performance liquid chromatography/Ultrafiltration centrifugation/Encapsulation rate/Methodology引用本文复制引用
出版年
2024
NETL
NSTL
万方数据