Determination of encapsulation rate of ruxolitinib solid lipid nanoparticles by ultrafiltration centrifugation-HPLC method
Objective To establish a method for determining the encapsulation rate of ruxolitinib solid lipid nanoparticles (Ru-SLN). Methods Ru-SLN was prepared using the melt emulsification-ultrasonic method,and the free drug and Ru-SLN were separated using ultrafiltration centrifugation. The Ru content was determined using HPLC,and the encapsulation rate was calculated accordingly. Results Ru had a good linear relationship within the concentration range of 4.08-408.00 μg/mL (r=0.9997),and its average recovery rate was 101.60％,with the RSD of 1.09％ (n=9). The ultrafiltration centrifugation selected ultrafiltration centrifuge tubes with a cut-off molecular weight of 10 kDa,centrifuged at 10961 × g for 10 minutes,and showed no significant membrane adsorption. The encapsulation rate of Ru-SLN was determined to be (97.01±1.23)％. Conclusion The combination of ultrafiltration centrifugation and HPLC method for determining the encapsulation efficiency of Ru-SLN is fast,convenient,and highly accurate,providing effective experimental guidance for the determination of Ru formulation content and encapsulation effciency.
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