Quality evaluation of Clematidis radix et rhizoma medicinal materials based on HPLC-QAMS multi-components quantification combined with OPLS-DA and weighted TOPSIS method
Objective To establish a method for simultaneous detection of 9 components in Clematidis radix et rhizoma from different producing areas,screen the differential markers affecting their quality,and to evaluate its quality difference. Methods Reflux extraction was performed on 16 batches of Clematidis radix et rhizoma from 8 provinces,and the extracts were detected by HPLC. The chemical identification model and weighted TOPSIS method were used to establish the quality evaluation model of Clematidis radix et rhizoma,and the quality difference was comprehensively evaluated. Results Clematichinenoside AR,huzhangoside D,clematichinenoside C,huzhangoside B,hederagenin,oleanolic acid,3,5,6,7,8,3',4'-heptamethoxyf lavone,hesperetin and formononetin showed good linear relationships within the ranges of 1.27-31.75,4.48-112.00,7.35-183.75,3.69-92.25,6.16-154.00,20.95-523.75,0.58-14.50,0.39-9.75 and 0.26-6.50 μg/mL (r>0.9990),respectively. The average recovery rate was 96.91％-100.12％,and the RSD was 0.71％-1.53％ (n=9). 16 batches of samples were grouped into 3 categories,and oleanolic acid,clematichinenoside C,clematichinenoside AR and hederagenin might be the main potential markers affecting the quality of Clematidis radix et rhizoma. The analysis results of the weighted TOPSIS method revealed that the closeness for evaluating the quality of 16 batches of Clematidis radix et rhizoma ranged from 0.0978 to 0.8182,with S13 achieving the highest value of 0.8182. Conclusion The method for quantitative analysis of 9 components in Clematidis radix et rhizoma is simple and accurate. Chemometrics and weighted TOPSIS method can be used to evaluate the quality difference.
Clematidis radix et rhizomaHigh-performance liquid chromatographyStechiometryPrincipal component analysisOrthogonal partial least squares discriminant analysisWeighted TOPSIS methodQuality evaluation
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