Comparison of extraction methods of main components of Rubiae radix et rhizoma and study of the changes before and after processing
Objective To compare the impacts of different extraction techniques on the primary components of Rubiae radix et rhizoma and investigate the variations in the major chemical constituents before and after processing. Methods The concentrations of purpurin and rubimaillin were assayed using the methods prescribed in the 2020 edition of the Chinese Pharmacopoeia (pharmacopoeia method) and an optimized method (improved method). The changes in these constituents were then compared and analyzed. Results The acid extraction method was adopted to simplify the alcohol extraction and acid hydrolysis process recommended by the pharmacopoeia method,leading to a more effcient and less time-consuming procedure. The results of methodological investigation showed that the stability of the solution was good within 24 h. The average recovery rates of purpurin and rubimaillin were in the range of 102.12％-104.95％ (with the RSD of 1.13％) and 93.78％-95.49％ (with the RSD of 0.80％),respectively (n=6). The determination results of pharmacopoeia method showed that the content of purpurin decreased,while the content of rubiarin increased after processing,which was not completely consistent with the actual trends. The improved method significantly improved the extraction effciency of purpurin,thus providing a more accurate reflection of the total purpurin content in Rubiae radix et rhizoma. Conclusion The established method is accurate,simple and effective,which provides reference for the reasonable formulation of inspection standards and quality control of madder.
Rubiae radix et rhizomaContent determinationPurpurinRubimaillinDecoction piece processingInspection standards
NETL
NSTL
万方数据
