羊水细胞原代和传代普通培养方法在产前诊断中应用比较分析
Analysis different methods between amniotic fluid cells primary culture and passage culture in prenatal diagnosis
夏燕 1段玲 1加米拉 1韩锐 1韩宁宁 1刘志娟 1武爽 1王晓岚1
作者信息
- 1. 新疆医科大学第一附属医院产前诊断中心,乌鲁木齐830054
- 折叠
摘要
目的 通过比较羊水细胞原代和传代普通培养方法,探讨和寻求实现羊水培养成功的方法.方法 77例羊水中35例羊水进行原代普通培养,42例羊水进行传代普通培养.结果 (1)羊水原代普通培养法较羊水传代普通培养法步骤简化,无需进行传代；接种至收获时间缩短(细胞克隆较多时)；制片获得羊水染色体较长,染色体形态好,便于分析；但细胞染色体分裂相相对有所减少.(2)羊水细胞克隆较少(＜ 10个克隆)时传代普通培养较原代普通培养法获得较多的细胞染色体分裂相,满足染色体计数和核型分析需要.结论 当羊水细胞克隆较多时,羊水原代普通培养法进行羊水细胞染色体产前诊断有明显优势,应优先选择；当羊水细胞克隆较少时,采用羊水传代普通培养法更能保障培养成功.
Abstract
Objective: To investigate a successful amniotic fluid cells culture method, compare different methods between amniotic fluid cells primary culture and passage culture. Methods: 35 of 77 amniotic fluid cells with primary culture, 42 amniotic fluid cells with passage culture. Results; ( 1) Primary culture was more simple, we cloud short the time and get the longer chromosomes in primary culture, but got the less chromosomes. (2 ) We cloud get the more chromosomes to analysis in passage culture, when the number of cell clones was less then 10. Conclusions; We should choose primary culture, when we could get the more cell clones; We should choose passage culture, when we could get the less cell clones.
关键词
羊水/普通培养/产前诊断Key words
Amniotic fluid/Cells cultivate/Prenatal diagnosis引用本文复制引用
出版年
2013
NETL
NSTL
维普
万方数据