Therapeutic effect and mechanism of paeonol on preeclampsia rats by inhibiting TLR4/NF-κB signaling pathway
Objective To investigate the therapeutic effect and mechanism of paeonol on preeclampsia rats by inhib-iting TLR4/NF-κB signaling pathway.Methods PE rat models were established and separated into Control group,Model group,paeonol low,medium and high dose groups(Pae-L,Pae-M and Pae-H groups),and paeonol high dose+lipopolysaccharide group(Pae-H+LPS group).Caudal sphygmomanometer was applied to measure the systolic blood pressure of rats.The number of embryos surviving,fetal and placental weights were recorded.ELISA was applied to detect the levels of urinary protein(UP),placental growth factor(PLGF),soluble fms-like tyrosine kinase-1(sFlt-1),and vascular endothelial growth factor(VEGF)levels,inflammatory factors,and oxidative stress-related indicators.H-E staining was applied to observe pathological changes in placental tissue.TUNEL was used to detect apoptosis in placental tissue cells.Western blot was used to detect apoptosis and TLR4/NF-κB signaling pathway related protein levels.Results Compared with the Control group,the placental tissue cells in the Model group showed increased swelling and vacuolization,and the infiltration of inflammatory cells and fibroid necrosis increased.The levels of SBP,UP,sFlt-1,TNF-α,IL-6,IFN-γ,ROS and MDA,cell apoptosis rate,and the expression of Bax,Caspase-3,TLR4,and NF-κB p65 proteins were obviously increased(P＜0.05),the fetal weight,the levels of PLGF,VEGF,SOD,and the expression of Bcl-2 protein were obviously reduced(P＜0.05).Compared with the Model group,the inflamma-tory infiltration of placental tissue in rats in the Pae-L group,Pae-M group,and Pae-H group decreased,and the number of new blood vessels increased;the levels of SBP,UP,sFlt-1,TNF-α,IL-6,IFN-γ,ROS and MDA,cell apoptosis rate,and the ex-pression of Bax,Caspase-3,TLR4,and NF-κB p65 proteins were obviously reduced(P＜0.05),the fetal weight,the levels of PLGF,VEGF,SOD and the expression of Bcl-2 protein were obviously increased(P＜0.05),and were dose-dependent.The TLR4 pathway inhibitor LPS reversed the protective effect of paeonol on PE rats.Conclusion The therapeutic effect of paeonol on preeclampsia rats may be related to the inhibition of TLR4/NF-κB signaling pathway.
paeonolcell surface receptor Toll like receptor 4/nuclear factor-κB signaling pathwaypreeclampsia
NETL
NSTL
万方数据
