Objective The aim of this study was to identify potential serum biomarkers of insulin resistance(IR)in polycystic ovary syndrome(PCOS)by comparing the serum protein expression levels between PCOS patients with and with-out insulin resistance(IR).Methods 193 patients with PCOS who were treated at Dongguan Traditional Chinese Medicine Hospital from January 2022 to December 2022 were divided into insulin resistance group(PCOS-IR,n=95)and non insulin resistance group(PCOS-NIR,n=98)based on insulin resistance steady-state model evaluation.Non PCOS patients in the same period were selected as healthy control group(n=34).Eight inflammatory markers were tested using ELISA:KLF,INF-γ,IL-2,IL-4,IL-6,IL-10,IL-22,TNF-α.Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS)was used to identify the differences in protein expression levels between PCOS-IR and PCOS-NIR groups.Results The levels of KLF,IL-4,IL-6 and TNF-α were the highest in the PCOS-IR group and the lowest in the healthy group,while the levels of INF-γ,IL-2,IL-10 and IL-22 were the lowest in the PCOS-IR group and the highest in the healthy group.Peptide mass spectrometry analysis showed that the relative peak intensities of 83 ion peaks were significantly different between the two groups(P＜0.05).PCA and PLS-DA analysis could clearly distinguish the healthy control group and the PCOS-IR group.Al-though PCOS group had a tendency to separate from the other two groups,there would be overlap.Conclusion This study found that there were differences in serum protein and polypeptide composition among PCOS group,PCOS-IR group and healthy control group,which had a good potential in the diagnosis or screening of PCOS-IR.
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