Neuroprotective effect of arbutin on neonatal rats with hypoxic ischemic brain damage by regulating the Gas6/Axl signaling pathway
Objective To investigate the neuroprotective effect of arbutin on neonatal rats with hypoxic ischemic brain damage(HIBD)by regulating the Gas6/Axl signaling pathway.Methods The Rice-Vannucci method was applied to construct a neonatal rat model of HIBD,48 newly born rats that were successfully modeled were randomly separated into a Model group,a low-dose arbutin group(50 mg/kg),a high-dose arbutin group(100 mg/kg),and a high-dose arbu-tin+Anticancer agent 109 group(100 mg/kg+3.00 mg/kg Gas6/Axl inhibitor),another 12 newborn rats were regarded as the NC group.The balance beam method was used to evaluate the behavior of each group of rats.Hematoxylin eosin(H-E)stain-ing was used to detect pathological damage in brain tissue.TTC staining method was used to detect the area of cerebral in-farction.ELISA was used to detect the expression of interleukin-6(IL-6),superoxide dismutase(SOD),malondialdehyde(MDA),and brain derived neurotrophic factor(BDNF)in serum.Western blot was used to detect the expression of Gas6,phosphorylated Axl(p-Axl),and Axl proteins in hippocampal tissue.Results Compared with the Model group,the behavioral scores of rats in the low-dose and high-dose arbutin groups decreased,and the brain tissue structure was more normal,the area of cerebral infarction in rats reduced,the expression of IL-6 and MDA in serum decreased,the expression of SOD and BDNF increased,the expression of Gas6 and Axl proteins increased,the expression of p-Axl protein decreased(P＜0.05).The results of the high-dose arbutin+Anticancer agent 109 group were opposite to those of the high-dose arbutin group,and the nerve damage in HIBD rats worsened(P＜0.05).Conclusion Arbutin may exert neuroprotective effects on neonatal rats with hypoxic ischemic brain damage by activating the Gas6/Axl signaling pathway.
万方数据
维普
