首页|不同邻苯二甲酸二乙基己基酯暴露时间、暴露剂量对新生鼠肺成熟的影响

不同邻苯二甲酸二乙基己基酯暴露时间、暴露剂量对新生鼠肺成熟的影响

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目的 在探讨不同邻苯二甲酸二乙基己基酯(DEHP)暴露时间、暴露剂量对新生鼠肺成熟的影响.方法 初生SD大鼠120只,将其随机分成4组各30只,即对照组、低剂量组、中剂量组及高剂量组.低剂量组给予DEHP 10 mg/kg,中剂量给予DEHP 100 mg/kg,高剂量组给予DEHP 750 mg/kg;对照组给予与DEHP给药组等体积的玉米油.各组在第14天及第21天时各随机取15只处死,取材肺部组织,应用于后续试验.H-E染色法评估肺组织病理变化;免疫组化法评估肺成熟相关蛋白水平;实时荧光定量PCR法检测MMP-9、TIMP-1及TGF-βmRNA的表达.结果 与对照组相比,DEHP暴露会导致新生鼠辐射状肺泡计数(RAC)减少、平均肺泡直径(MAD)和肺泡平均截距(MLI)增大.DEHP暴露2周导致肺泡损伤,延长暴露时间至3周肺泡损伤加重,且剂量越大肺泡损伤越严重.在mRNA水平和蛋白水平上,随着DEHP剂量的升高MMP-9和TIMP-1的表达逐渐上升,TGF-β的表达逐渐下降,此外,随着DEHP暴露时间的增加MMP-9和TIMP-1的表达逐渐上升,TGF-β的表达逐渐下降.结论 邻苯二甲酸二乙基己基酯暴露会影响新生鼠肺的发育,且暴露的剂量及时间增加会导致新生鼠肺发育迟缓加重.
Effects of exposure time and dose of diethylhexyl phthalate on lung maturation in neonatal rats
Objective To investigate the effects of exposure time and dose of diethylhexyl phthalate(DEHP)on lung maturation in neonatal rats.Methods A total of 120 SD rats were stochastic divided into 4 groups with 30 rats in each group,which were control,low dose,medium dose and high dose group,respectively.The low-dose group was given 10 mg/kg DEHP,the medium-dose group was given 100 mg/kg DEHP,and the high-dose group was given 750 mg/kg DEHP.The amount of corn oil administered to the control group was the same as that given to the DEHP group.On the 14th day and the 21st day,15 rats in each group were randomly sacrificed,and lung tissues were taken for subsequent experiments.H-E staining was used to evaluate the pathological changes of lung tissue.The levels of lung maturation related proteins were evaluated by immuno-histochemistry.The expression of lung maturation related proteins was detected by immunohistochemical staining.Real-time fluorescence quantitative PCR was used to measure MMP-9,TIMP-1 and TGF-β mRNA expression levels.Results DEHP exposure resulted in decreased radial alveolar count(RAC)and increased mean alveolar diameter(MAD)and mean intercept(MLI)in newborn rats.Exposure to DEHP resulted in alveolar injury for 2 weeks,and prolonged exposure time to 3 weeks aggravated the alveolar injury,and the higher the dose,the more serious the alveolar injury.In terms of mRNA level and pro-tein level,with the increase of DEHP dose,the expression of MMP-9 and TIMP-1 increased gradually,and the expression of TGF-β decreased gradually.In addition,with the increase of DEHP exposure time,the expression of MMP-9 and TIMP-1 in-creased gradually,and the expression of TGF-β decreased gradually.Conclusion Exposure to diethylhexyl phthalate can affect the lung development of neonatal rats,and the increase of exposure dose and time can aggravate the lung development delay of neonatal rats.

diethylhexyl phthalatenewborn micelung maturation

应燕芬、陈丽华、施玲玲

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浙江省台州医院新生儿科,浙江临海 317099

邻苯二甲酸二乙基己基酯 新生鼠 肺成熟

台州市科技计划

XM20190627

2024

中国优生与遗传杂志
中国优生科学协会

中国优生与遗传杂志

CSTPCD
影响因子:0.527
ISSN:1006-9534
年,卷(期):2024.32(2)
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