The effect of lncRNA SNHG6 regulating miR-1297 expression on the biological behavior of cervical cancer cells
Objective To analyze the expression levels of lncRNA SNHG6 and miR-1297 in cervical cancer tissues and cell lines,and to explore their relationship with clinical pathological characteristics of patients and the biological behavior of cervical cancer cells.Methods qRT-PCR was used to detect the expression of lncRNA SNHG6 and miR-1297 in 90 cases of cervical cancer tissues,paracancerous tissues,human biological immortality cervical epithelial cells(H8 cells)and cervical cancer cell lines,the relationship between the expression of lncRNA SNHG6 and miR-1297 in cervical cancer tissue and clinical pathological indicators was analyzed.HCC-94 cells were divided into control group,si-NC group,si-SNHG6 group,si-SNHG6+anti-miR-NC group,and si-SNHG6+anti-miR-1297 group,qRT-PCR was applied to detect the expression of lncRNA SNHG6 and miR-1297 in cells.Cell proliferation,migration,invasion and apoptosis were detected.Western blot was applied to detect the expression of proliferating cell nuclear antigen(PCNA),matrix metalloproteinase-2(MMP-2),Bcl-2 associated X protein(Bax),and anti apoptosis factor B cell lymphoblastoma 2(Bcl-2)protein,and double luciferase reporter gene experiment was applied to verify the targeted regulation relationship between lncRNA SNHG6 and miR-1297.Results In cervical cancer tissues and cells,the expression level of lncRNA SNHG6 increased,while the expression level of miR-1297 decreased(P＜0.05).The expression level of lncRNA SNHG6 increased and the expression level of miR-1297 decreased in tissues of cervical cancer patients with clinical stage ≥ Ⅲ,low differentiation,and lymph node metastasis(P＜0.05).Knock-ing-down the expression of lncRNA SNHG6 was able to obviously inhibit the proliferation,migration,and invasion of HCC-94 cells,and promote cell apoptosis(P＜0.05).Inhibiting the expression of miR-1297 was able to reverse the inhibitory effect of knocking down lncRNA SNHG6 expression on the proliferation,migration,and invasion of HCC-94 cells,and the promoting effect on cell apoptosis(P＜0.05).The double luciferase reporter gene experiment confirmed that there was a tar-geted regulatory relationship between lncRNA SNHG6 and miR-1297.Conclusion lncRNA SNHG6 is up-regulated and miR-1297 is down-regulated in cervical cancer tissues and cells,they are closely related to tumor staging,tissue differentiation,and lymph node metastasis.Knocking-down lncRNA SNHG6 may up-regulate miR-1297 to inhibit the proliferation,migration,and invasion of cervical cancer cells,and promote their apoptosis.
cervical cancerlncRNA SNHG6/miR-1297proliferationmigration and invasionapoptosis
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维普
