Effect of galangin on hyperoxia-induced lung injury in premature rats by regulating the AMPK/SIRT1/PGC-1α signaling pathway
Objective To investigate the effect of galangin(Ga)on hyperoxia-induced lung injury and adenylate ac-tivated protein kinase(AMPK)/silent information regulator factor 1(SIRT1)/peroxisome proliferator-activated receptor γcoactivator 1α(PGC-1α)pathway in premature rats.Methods The hyperoxia induced lung injury model of premature rats was established.The rats were divided into Control group,Model group,low and high dose Ga groups(Ga-L group,Ga-H group),and Ga-H+AMPK inhibitor Dorsomorphin group(Ga-H+Dors group);the wet to dry weight ratio of rat lungs was detected;H-E staining was used to observe pathological changes in lung tissue.ELISA was used to detect tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)levels in bronchoalveolar lavage fluid(BALF).The kits were used to detect levels of super-oxide dismutase(SOD),malondialdehyde(MDA),and glutathione(GSH).TUNEL staining was used to detect apoptosis in lung tissue cells.Western blot was used to detect the expression of BCL2-associated X protein(Bax),B-cell lymphoma-2(Bcl-2),AMPK,SIRT1,and PGC-1α proteins.Results Compared with Control group,the ratio of lung wet weight to dry weight,TNF-α,IL-6,MDA,cell apoptosis rate,and the level of Bax in Model group were increased,the levels of SOD,GSH,Bcl-2,p-AMPK/AMPK,SIRT1,and PGC-1α were reduced(P<0.05);compared with Model group,the ratio of lung wet weight to dry weight,TNF-α,IL-6,MDA,cell apoptosis rate,and the expression level of Bax in Ga-L and Ga-H groups were reduced,the levels of SOD,GSH,Bcl-2,p-AMPK/AMPK,SIRT1,and PGC-1α were increased(P<0.05);Dorsomorphin attenuated the ameliorative effect of Ga-H on lung injury in hyperoxy-induced preterm rats.Conclusion Ga may inhibit inflammation,oxi-dative stress and apoptosis by activating the AMPK/SIRT1/PGC-1α pathway,and alleviate lung injury in premature rats in-duced by hyperoxia.
galanginadenylate activated protein kinase(AMPK)/silent information regulator factor 1(SIRT1)/peroxisome proliferator-activated receptor γ coactivator 1α(PGC-1α)signaling pathwayhyperoxiapremature ratslung in-jury