Clinical application of serum miR-665 and miR-302e in early diagnosis of ovarian cancer
Objective To explore the clinical application of serum miRNA(miR)-665 and miR-302e expression in the early diagnosis of ovarian cancer.Methods From March 2020 to December 2022,90 ovarian cancer patients(ovarian cancer group)and 85 healthy individuals(control group)who received treatment in The Second Affiliated Hospital of the Chinese People's Liberation Army Air Force Medical University were collected,Real-time quantitative fluorescence polymerase chain reaction(qRT-PCR)was applied to detect the expression of miR-665 and miR-302e in serum.Pearson method,receiver oper-ating characteristic and Logistic regression were applied to analyze the correlation between serum miR-665 and miR-302e levels,the diagnostic value of the two levels in serum for ovarian cancer,and the factors affecting the occurrence of ovarian cancer,the relationship between serum miR-665 and miR-302e levels and clinical pathology of ovarian cancer patients was analyzed.Results Compared with the control group,the serum levels of miR-665 and miR-302e in the ovarian cancer group showed a statistically obvious decrease(P＜0.05).There was a positive correlation between serum miR-665 and miR-302e levels in ovarian cancer patients(P＜0.001).The area under the curve,sensitivity,and specificity of serum miR-665 and miR-302e in the diagnosis of ovarian cancer patients were 0.779,0.877,77.78％,72.22％,70.59％,and 87.06％,respectively,the optimal cutoff value was 0.960 and 0.880,respectively.The area under the curve for the combined diagnosis of ovarian cancer was 0.900,with a sensitivity of 67.78％and a specificity of 96.47％.Logistic regression analysis showed that the influencing factors for the occurrence of ovarian cancer in patients were serum levels of miR-665 and miR-302e(P＜0.05).Conclusion The expression of miR-665 and miR-302e in serum can be used for early diagnosis of ovarian cancer,and the combined diagnosis is more effective.
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