MiR-519e-5p/SAT1 axis regulates the occurrence and development of endometriosis by mediating ferroptosis signal pathway
Objective To investigate the effect of microRNA-519e-5p(miR-519e-5p)on endometriosis by targeting the regulation of spermine N1-acetyltransferase 1(SAT1).Methods From January 2023 to June 2023,the ectopic endo-metrium tissues of patients with ovarian endometriosis hospitalized in Zhoupu Hospital Affiliated to Shanghai University of Medicine & Health Sciences were collected,and the expression of miR-519e-5p and SAT1 proteins was detected;cells were isolated from ectopic lesion tissue and separated into Control group,anti-miR-NC group,anti-miR-519e-5p group,anti-miR-519e-5p+NC group,and anti-miR-519e-5p+sh-SATl group,luciferase activity,cell activity,numbers of live and dead cells,Fe2+,ROS,GSH,and MDA were detected separately.Prussian blue staining was applied to observe the distribution of iron particles.RT-qPCR was applied to detect miR-519e-5p and SAT1 mRNA expression;and Western blot was applied to detect the expression of SAT1,GPX4,SLC7A11,MUC1,and ACSL4 proteins.Results Compared with normal endometrium,miR-519e-5p obviously increased and SAT1 expression obviously decreased in ectopic endometrial tissue(P＜0.05).The de-tection results of luciferase reporter gene showed that miR-519e-5p had a targeting relationship with SAT1.After inhibiting miR-519e-5p,cell activity,GSH,GPX4,SLC7A11,MUC1 were obviously reduced,while the average optical density of Fe,Fe2+,MDA,ROS,and ACSL4 were obviously increased(P＜0.05).Silencing SAT1 and inhibiting miR-519e-5p expression obviously increased cell activity,GSH,GPX4,SLC7A11,and MUC1,and obviously decreased the average optical density of Fe,Fe2+,MDA,ROS,and ACSL4(P＜0.05).Conclusion The expression of miR-519e-5p is up-regulated in endometriosis.Inhibiting the expression of miR-519e-5p was able to activate the ferroptosis signal pathway and inhibit the occurrence of endometriosis.
NETL
NSTL
万方数据
