Objective To investigate the effect of lncRNA CRNDE on the proliferation,apoptosis,and invasion abilities of HEC-1A cells.Methods The expression difference of CRNDE in hESC cells and HEC-1A cells was compared with RT-qPCR.After transfecting HEC-1A cells with shRNA-CRNDE lentivirus,its cell proliferation,migration and invasion abilities,and cell apoptosis levels were detected by CCK-8,transwell migration experiment,matrigel invasion experiment,and flow cytometry.Results The CRNDE expression level,proliferation,migration and invasion abilities of HEC-1A cells were higher than those of hESCs.After CRNDE was inhibited,these abilities were significantly weakened,but still slightly higher than those of hESC cells.At the same time,CRNDE silencing also led to a significant increase in the apoptosis rate of HEC-1A cells.Conclusion CRNDE is highly expressed in HEC-1A cells and is directly related to cell proliferation,migration and invasion ability,and apoptosis level,indicating that it has the potential to be a diagnostic and therapeutic target for endometrial cancer.
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