Effect of erianin on malignant progression of ovarian cancer cells through CCL2-CCR2 signaling axis
Objective To investigate the regulatory effect of erianin(ER)on the malignant progression of ovarian cancer cells and its regulatory mechanism on the CCL2-CCR2 signaling axis.Methods Human ovarian cancer cells SKOV3 were cultured and divided into SKOV3 group,ER low concentration(ER-L)group,ER medium concentration(ER-M)group,ER high concentration(ER-H)group,and ER-H+pcDNA-CCL2 group.CCK-8 method and clone plate assay were used to detect the proliferation activity of cells.Transwell experiment was used to detect the migration and invasion ability of cells;flow cytometry was used to detect the apoptosis rate of cells.Western blot was used to detect the expression of CCL2-CCR2 signaling axis related proteins of cells.Results Compared with the SKOV3 group,the cell survival rate,the number of clones formed,the number of migrating and invading cells and the expression levels of CCL2 and CCR2 in ER-L,ER-M and ER-H groups were decreased(P＜0.05),and the cell apoptosis rate was increased(P＜0.05).Overexpression of CCL2 weakened the inhibitory effect of high concentration ER on malignant progression of SKOV3 cells(P＜0.05).Conclusion ER can inhibit the malignant progression of ovarian cancer cells,and its mechanism of action may be related to the inhibition of the CCL2-CCR2 signaling axis.
erianinCCL2-CCR2 signal axisovarian cancerproliferationmigration
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