Expression and clinical significance of miR-125b-1-3p and NR3C1 in patients with GDM based on RBM analysis
Objective To explore the potential biomarkers of gestational diabetes mellitus(GDM),analyze the ex-pression and clinical significance of miR-125b-1-3p and NR3C1 in GDM patients,and provide a theoretical basis for the di-agnosis and intervention of GDM.Methods The target dataset was found in Gene Expression Ominbus(GEO)and the differ-entially expressed miRNA and mRNA of GDM were screened.Then,Gene Ontology(GO)and The Kyoto Encyclopedia of Genes and Genomes(KEGG)Database were enriched.We mapped the network of protein-protein interactions(PPI)to screen and visually analyze key GDM genes.The expression levels of miR-125b-1-3p and NR3C1 were verified by GEO database and RT-qPCR.The effect of miR-125b-1-3p on direct binding and transcriptional activity of target gene NR3C1 was deter-mined by dual luciferase reporter gene.Results The GSE186883 dataset of plasma was retrieved,and 267 differentially ex-pressed miRNA were obtained,all of which were down-regulated miRNA.An independent GDM placental cord blood mi-croarray dataset GSE203346 was obtained from GEO database,and 477 differentially expressed genes were obtained,includ-ing 295 down-regulated genes and 182 up-regulated genes.KEGG analysis showed that differentially expressed genes were mainly associated with toll-like receptor signaling pathway,natural killer cell-mediated cytotoxicity,type Ⅰ diabetes,and NOD-like receptor signaling pathway.An independent placental microarray dataset GSE203346 for gestational diabetes mel-litus was obtained from the GEO database,with a total of 9585 differentially expressed genes,including 9245 down-regulated genes and 340 up-regulated genes.24 miR-125b-l-3p target genes were predicted in miRDB,13 in miRTarBase,and 489 in TargetScan.The miR-125b-1-3p target gene NR3C1 was significantly associated with the disease.Compared with normal pregnant women,the expression level of miR-125b-1-3p was decreased and the expression level of NR3C1 was increased in GDM patients(P＜0.05),and the level of NR3C1 was negatively correlated with the expression of miR-125b-1-3p(P＜0.05).Dual luciferase reporter gene assay suggested that miR-125b-1-3p inhibited NR3C1 expression through 3'-UTR of target NR3C1 mRNA.Conclusion Through bioinformatics analysis,miR-125b-1-3p and NR3C1 can provide a theoretical basis for seeking potential molecular markers in GDM.
miR-125b-1-3pNR3C1gestational diabetesbioinformatics and the analysis
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