IL-35、Beclin-1和LDH在乳腺癌患者中表达水平研究
Expression of IL-35,Beclin-1,and LDH in breast cancer patients
宦宇 1齐结华 1吴静娟 1金剑 2宋毅 2张春利 1蒋佩佩 1吴守乐 1蔡徐山1
作者信息
- 1. 上海市嘉定区妇幼保健院检验科,上海 201821
- 2. 上海市嘉定区妇幼保健院病理科,上海 201821
- 折叠
摘要
目的 探讨白细胞介素35(IL-35)、Beclin-1和乳酸脱氢酶(LDH)在乳腺癌患者外周血和组织中的表达情况及相关性.方法 选取2022年4-9月在上海市嘉定区妇幼保健院诊治的原发性乳腺癌的女性患者共44例,对照组46例,ELISA测定外周血血浆中IL-35和Beclin-1水平;全自动生化分析仪上分析血清LDH水平;免疫组化分析EBI3、Beclin-1和LDHA蛋白抗体在组织中的表达水平.结果 与对照组相比,外周血中IL-35[(19.30±3.63)μg/mL]和 LDH[(191.50±43.07)U/L]水平高表达(P＜0.05),Beclin-1[(2.39±0.517)ng/mL]低表达(P＜0.05);相关性分析显示IL-35、LDH与Beclin-1分别都呈负相关,而IL-35与LDH之间呈正相关.ROC曲线分析显示,各项外周血指标联合检测诊断的 AUC 值为 0.861,高于 IL-35(0.768)、Beclin-1(0.733)及 LDH(0.657)单一诊断 AUC 值(P＜0.05).EBI3特异性抗体间接表示的IL-35,LDHA间接表示的LDH以及Beclin-1蛋白抗体的免疫组化结果显示,与癌旁组织相比,乳腺癌组织中IL-35和LDH蛋白水平高表达,Beclin-1低表达.结论 乳腺癌患者外周血和组织中IL-35和LDH高表达,Beclin-1低表达,IL-35可能与自噬水平呈负相关,与糖代谢水平呈正相关.
Abstract
Objective To investigate the expression and correlation of IL-35,Beclin-1,and LDH in peripheral blood and tissues of breast cancer patients.Methods From April to September 2022,44 female patients with primary breast cancer who were diagnosed and treated in Shanghai Jiading Maternal and Child Health Hospital were selected,and 46 women in the control group were selected.The levels of IL-35 and Beclin-1 in peripheral blood plasma were measured by ELISA.LDH levels of serum were analyzed on a fully automated biochemical analyzer.The levels of EBI3,Beclin-1,and LDHA protein expressions in tissues were detected by Immunohistochemical.Results Compared with the control group,IL-35[(19.30±3.63)μg/mL]and LDH[(191.50±43.07)U/L]in peripheral blood were higher expressed(P＜0.05),while Beclin-1[(2.39±0.517)ng/mL]levels were lower(P＜0.05).Correlation analysis shows that IL-35,LDH,were both negatively correlated with Beclin-1,while IL-35 and LDH were positively correlated with each other.According to the ROC curve,the AUC value diag-nosed by the combined detection of various peripheral blood indicators was 0.861,which was higher than the three single diagnoses(P＜0.05).Immunohistochemical results of EBI3,Beclin-1,and LDHA,protein antibodies showed that compared with adjacent tissues,the levels of IL-35 and LDH protein in breast cancer tissues were higher,and the levels of Beclin-1 pro-tein were lower.Conclusion In peripheral blood and tissues of breast cancer patients,IL-35 and LDH had a higher expression,while Beclin-1 had a lower expression.IL-35 may be negatively correlated with autophagy level,and positively correlated with glucose metabolism level.
关键词
白细胞介素35/Beclin-1/乳酸脱氢酶/乳腺癌/免疫组化Key words
interleukin-35(IL-35)/Beclin-1/lactate dehydrogenase(LDH)/breast cancer/immunohistochemical(IHC)引用本文复制引用
基金项目
上海市嘉定区自然科学基金(JDKW-2020-0041)
嘉定区医学重点学科建设项目(第五批)(2020-jdyxzdfcxk-03)
出版年
2024
NETL
NSTL
万方数据