Objective To investigate the effects of lncRNA NEAT1 on the regulation of cervical cancer cell migration,invasion,apoptosis and EMT through the miR-802/TRPM7 axis.Methods Siha cells were divided into Control group(without transfection),si-NC group(transfection si-NC),si-NEAT1 group(transfection si-NEAT1),si-NEAT1+in-miR-802 group(transfection si-NEAT1+miR-802inhibitor)and si-NEAT1+TRPM7 group(transfected si-NEAT1+pcDNA3.1-TRPM7),miR-NC group(transfected miR-NC)and miR-802mimics group(transfected miR-802mimics),NEAT1,miR-802 and TRPM7 in cervical cancer tissues and cell lines were detected by qRT-PCR mRNA expression,cell invasion was detected by Transwell assay,cell migration was detected by cell scratch assay,cell apoptosis was detected by flow cytometry,E-cadherin and Vimentin protein expressions were detected by Western blot,and binding sites between NEAT1 and miR-802,and between-miR-802 and TRPM7 were predicted by StarBase and Targetscan databases.Dual luciferase reports detected the targeting re-lationship between NEAT1 and miR-802 and between miR-802 and TRPM7.Results Compared with adjacent tissues,the expression of NEAT1 and TRPM7 in cancer tissues was obvious increased(P＜0.05),while the expression of miR-802 was obviousreduced(P＜0.05).Compared with normal cervical HaCat cells,the expressions of NEAT1 and TRPM7 in cervical cancer cell lines Siha,HeLa,C33A,ME 180,HEK-293T and Caski cells were obviously increased,while the expression of miR-802 was obviously decreased(P＜0.05).Compared with Control group and si-NC group,the scratch healing rate and in-vasion number of cervical cancer Siha cells in si-NEAT1 group were obvious decreased,and the apoptosis rate was obviously increased(P＜0.05).Compared with si-NEAT1 group,the scratch healing rate and invasion number of cervical cancer Siha cells in si-NEAT1+in-Mir-802 group and si-NEAT1+TRPM7 group were obviously increased,and the apoptosis rate was obviously decreased(P＜0.05).Compared with the Control group and the si-NC group,the expression of E-cadherin protein in cervical cancer Siha cells in the si-NEAT1 group was obviousincreased,and the expression of Vimentin protein was decreased(P＜0.05).The expression of E-cadherin protein in cervical cancer Siha cells of Si-Neat1+in-miR-802 group and si-NEAT1+TRPM7 group was obviously decreased,and the expression of Vimentin protein was obviousincreased(P＜0.05).Conclusion NEAT1 is highly expressed in cervical cancer,and reducing its expression can inhibit the migration,invasion and EMT of cervical cancer cells,and promote apoptosis.NEAT1 may play a role in regulating the expression of TRPM7 by in-teracting with miR-802.
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