Sevoflurane anesthesia during pregnancy regulates the effects of miR-206 on cognitive dysfunction in offspring rats
Objective To investigate the mechanism of sevoflurane anesthetized rats during pregnancy through regu-lating miR-206 on the cognitive dysfunction of offspring.Methods The progenies of the pregnant rats in the control group were divided into the control group,and the progenies of the anesthesia group were randomly divided into the anesthesia group,the anesthesia+NC inhibitor group,and the anesthesia+miR-206 inhibitor group,with 8 rats in each group.Morris water maze test was used to test the learning and memory ability of the rats,and Nissl bodies in the hippocampus of each group were de-tected by Nissl staining.The mRNA expression levels of miR-206 and BDNF were detected by RT-qPCR,and the protein ex-pression of BDNF was analyzed by Western blot.IL-6,IL-1β and TNF-α activities were detected by ELISA.Results Com-pared with the control group,the escape latency and the times of crossing the platform of the offspring rats in the anesthesia group and the anesthesia+NC inhibitor group were significantly reduced,the relative expression level of miR-206 mRNA was significantly increased,the Nissl body was sparse,and the expression levels of IL-6,IL-1β and TNF-α were significantly in-creased(P＜0.01).Compared with the anesthesia+NC inhibitor group,the escape latency and platform crossing times of the anesthesia+miR-206 inhibitor group were significantly increased,the relative expression level of miR-206 mRNA was sig-nificantly decreased,the hippocampus was intact,the boundary of the Nishi body was clear,and the expression levels of IL-6,IL-1β and TNF-α were significantly decreased(P＜0.05).Target prediction and luciferase assay showed that BDNF was the direct target of miR-206 in HEK-293 cells and was negatively correlated with regulation.Conclusion Sevoflurane anesthesia during pregnancy can cause cognitive dysfunction in offspring rats,and can improve cognitive dysfunction by inhibiting the expression of BDNF by inhibiting miR-206.
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