首页|茯苓多糖调节SIRT1/AMPK信号通路对多囊卵巢综合征大鼠卵巢功能的影响

茯苓多糖调节SIRT1/AMPK信号通路对多囊卵巢综合征大鼠卵巢功能的影响

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目的 探究茯苓多糖(PPC)调节沉默信息调节因子1(SIRT1)/单磷酸腺苷活化蛋白激酶(AMPK)信号通路对多囊卵巢综合征(PCOS)大鼠卵巢功能的影响.方法 将大鼠分为对照组,PCOS组,PPC低、中、高剂量(PPC-L、PPC-M、PPC-H)组,EX527组(PPC-H+SIRT1/AMPK通路抑制剂),除对照组外,均采用来曲唑联合高脂饮食复制PCOS大鼠模型.血糖仪测量空腹血糖(FBG);ELISA法检测血清空腹胰岛素(FINS)、肿瘤坏死因子α(TNF-α)、白细胞介素-1β(IL-1β)、睾酮(T)、卵泡刺激素(FSH)、促黄体生成素(LH)水平;H-E染色观察卵巢形态变化;Western blot检测SIRT1/AMPK信号通路蛋白表达.结果 与对照组比较,PCOS组FBG、FINS、HOMA-IR、T、LH、LH/FSH水平、TNF-α、IL-1β水平、卵巢指数升高,FSH水平、成熟卵泡数、黄体数、SIRT1、p-AMPK/AMPK表达降低(P<0.05);与 PCOS 组比较,PPC-L、PPC-M、PPC-H 组 FBG、FINS、HOMA-IR、T、LH、LH/FSH 水平、TNF-α、IL-1β水平、卵巢指数降低,FSH水平、成熟卵泡数、黄体数、SIRT1、p-AMPK/AMPK表达升高(P<0.05);EX527可显著减轻PPC对PCOS大鼠卵巢功能的改善作用(P<0.05).结论 PPC可减轻炎症反应,调节激素水平,改善PCOS大鼠卵巢功能,其作用机制可能与SIRT1/AMPK信号通路激活有关.
Effect of Poria cocos polysaccharides on ovarian function in polycystic ovary syndrome rats by regulating the SIRT1/AMPK signaling pathway
Objective To investigate the effect of Poria cocos polysaccharides(PPC)on ovarian function in polycys-tic ovary syndrome(PCOS)rats by regulating the silencing information regulatory factor 1(SIRT1)/adenosine monophos-phate-activated protein kinase(AMPK)signaling pathway.Methods Rats were separated into control group,PCOS group,PPC low,medium,and high dose(PPC-L,PPC-M,PPC-H)groups,and EX527 group(PPC-H+SIRT1/AMPK pathway inhibi-tor),except for the control group,other groups were used to replicate PCOS rat models using triazole combined with a high-fat diet.Blood glucose meter was applied to measure fasting blood glucose(FBG).ELISA method was applied to detect the levels of fasting insulin(FINS),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),testosterone(T),follicle stimulating hor-mone(FSH),and luteinizing hormone(LH)in serum.HE staining was applied to observe the morphological changes of ova-ries.Western blot was applied to detect the expression of SIRT1/AMPK signaling pathway proteins.Results Compared with the control group,the levels of FBG,FINS,HOMA-IR,T,LH,LH/FSH,TNF-α,IL-1β,and ovarian index in the PCOS group increased,the levels of FSH,number of mature follicles,corpus luteum,and expression of SIRT1,p-AMPK/AMPK decreased(P<0.05).Compared with the PCOS group,the levels of FBG,FINS,HOMA-IR,T,LH,LH/FSH,TNF-α,IL-1β,and ovarian index decreased in the PPC-L,PPC-M,and PPC-H groups,the levels of FSH,number of mature follicles,corpus luteum,and expression of SIRT1,p-AMPK/AMPK increased(P<0.05).EX527 was able to greatly alleviate the improvement effect of PPC on ovarian function in PCOS rats(P<0.05).Conclusion PPC can alleviate inflammatory response,regulate hormone level,and improve ovarian function in PCOS rats,which mechanism of action may be related to the activation of the SIRT1/AMPK sig-naling pathway.

Poria cocos polysaccharidesSIRT1/AMPK signaling pathwaypolycystic ovary syndromeovarian function

瞿谆、马惠荣、冯丹、刘佳、丑丹、黄艳辉

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武汉市中医医院妇科,湖北武汉 430000

河北中医药大学,河北石家庄 050300

茯苓多糖 SIRT1/AMPK信号通路 多囊卵巢综合征 卵巢功能

武汉市卫健委医学科研项目武汉市卫健委医学科研项目河北省自然科学基金武汉市中医药科研项目

WZ19A09WZ21C03H2022423334WZ22A10

2024

中国优生与遗传杂志
中国优生科学协会

中国优生与遗传杂志

CSTPCD
影响因子:0.527
ISSN:1006-9534
年,卷(期):2024.32(6)