Effect of Poria cocos polysaccharides on ovarian function in polycystic ovary syndrome rats by regulating the SIRT1/AMPK signaling pathway
Objective To investigate the effect of Poria cocos polysaccharides(PPC)on ovarian function in polycys-tic ovary syndrome(PCOS)rats by regulating the silencing information regulatory factor 1(SIRT1)/adenosine monophos-phate-activated protein kinase(AMPK)signaling pathway.Methods Rats were separated into control group,PCOS group,PPC low,medium,and high dose(PPC-L,PPC-M,PPC-H)groups,and EX527 group(PPC-H+SIRT1/AMPK pathway inhibi-tor),except for the control group,other groups were used to replicate PCOS rat models using triazole combined with a high-fat diet.Blood glucose meter was applied to measure fasting blood glucose(FBG).ELISA method was applied to detect the levels of fasting insulin(FINS),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),testosterone(T),follicle stimulating hor-mone(FSH),and luteinizing hormone(LH)in serum.HE staining was applied to observe the morphological changes of ova-ries.Western blot was applied to detect the expression of SIRT1/AMPK signaling pathway proteins.Results Compared with the control group,the levels of FBG,FINS,HOMA-IR,T,LH,LH/FSH,TNF-α,IL-1β,and ovarian index in the PCOS group increased,the levels of FSH,number of mature follicles,corpus luteum,and expression of SIRT1,p-AMPK/AMPK decreased(P<0.05).Compared with the PCOS group,the levels of FBG,FINS,HOMA-IR,T,LH,LH/FSH,TNF-α,IL-1β,and ovarian index decreased in the PPC-L,PPC-M,and PPC-H groups,the levels of FSH,number of mature follicles,corpus luteum,and expression of SIRT1,p-AMPK/AMPK increased(P<0.05).EX527 was able to greatly alleviate the improvement effect of PPC on ovarian function in PCOS rats(P<0.05).Conclusion PPC can alleviate inflammatory response,regulate hormone level,and improve ovarian function in PCOS rats,which mechanism of action may be related to the activation of the SIRT1/AMPK sig-naling pathway.
Poria cocos polysaccharidesSIRT1/AMPK signaling pathwaypolycystic ovary syndromeovarian function