Effects of sufentanil on proliferation,migration,and invasion of ovarian cancer cells by regulating the Notch-1/Hes signaling pathway
Objective To investigate the effects of sufentanil(SUF)on ovarian cancer cell proliferation,migration and invasion,and to analyze whether the mechanism is related to the Notch-1/Hes signaling pathway.Methods SKOV3 cells were divided into control group(untreated),low-dose SUF group(L-SUF group,0.01 μmol/L),medium-dose SUF group(M-SUF group,0.1 μmol/L),high-dose SUF group(H-SUF group,1 μmol/L),DAPT group(5 μmol/L Notch-l/Hes signaling pathway blocker DAPT),and H-SUF+Jagged1 group(1 μmol/L SUF and 400 μg/L Notch-1/Hes signaling pathway agonist Jagged1).CCK-8 kit to detect SKOV3 cell activity,flow cytometry to detect SKOV3 cell apoptosis,scratch assay to detect SKOV3 cell migration ability,Transwell assay to detect SKOV3 cell invasion ability,Western blot to detect the expression of Notch 1,Notch 2,Notch 3,E-cadherin,N-cadherin,Snail,Hes-1,Hes-5 proteins.Results Compared with the control group,OD value,number of invading cells,scratch healing rate,Snail,N-cadherin,Hes,and Notch-1 levels were lower in the L-SUF,M-SUF,H-SUF,and DAPT groups(P＜0.05).Apoptosis rate,and E-cadherin levels were higher in the L-SUF,M-SUF,H-SUF,and DAPT groups(P＜0.05).And compared with the H-SUF group,OD value,number of invading cells,scratch healing rate,Snail,N-cadherin,Hes,and Notch-1 levels were higher in H-SUF+Jagged1 group(P＜0.05).Apoptosis rate,E-cadherin levels were lower(P＜0.05).Conclusion SUF may promote ovarian cancer cell apoptosis by inhibiting the Notch-1/Hes signaling pathway,which in turn inhibits ovarian cancer cell proliferation,migration and invasion.
sufentanilNotch-1/Hes signaling pathwayovarian cancerproliferationmigration and invasion
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