Expression and clinical significance of miR-142-3p and miR-598-3p in ovarian benign and malignant tumor tissues
Objective To investigate the expression of microRNAs(miR)-142-3p and miR-598-3p in ovarian epithe-lial carcinoma(EOC)and benign ovarian tumor tissues,and the clinical significance of miR-142-3p and miR-598-3p in the differential diagnosis of EOC.Methods Patients with EOC or benign ovarian tumors who underwent surgery in Tangshan Maternal and Child Health Hospital from January 2021 to May 2023 were selected as the research objects,and miRNA se-quencing and cancer genome atlas(TCGA)analysis were performed in the training set tissue samples to screen out the differ-entially expressed miRNAs,and further verified in the validation set tissues,the expression of miRNAs was determined by quantitative reverse transcription polymerase chain reaction(qRT-PCR),and the ability of tissue miRNAs to identify benign and malignant ovarian tumors was analyzed by the receiver operator characteristic(ROC)curve.Results In training set,33 miRNAs were differentially expressed between the two groups(P＜0.01).miR-142-3p,miR-598-3p,miR-181b-5p,miR-19a-3p,miR-21-3p,and miR-55b-3p were also differentially expressed between tumors and adjacent normal tissues in TCGA ovarian cancer samples.In validation set,it was found that the levels of miR-142-3p and miR-598-3p in EOC tissues were lower than those in patients with benign ovarian tumors(P＜0.05).Low expression of miR-142-3p(P=0.001)and miR-598-3p(P=0.013)in EOC tissues was associated with more severe FIGO stage.The ROC curves showed that the area under the curve(AUC)of miR-142-3p and miR-598-3p in the differential diagnosis of EOC were 0.629 and 0.695,respectively.There is a synergistic effect in the combined diagnosis of miR-142-3p and miR-598-3p(AUC=0.802).In addition,the 2-miRNA combination(miR-142-3p+miR-598-3p)had a synergistic effect on the differential diagnosis AUC of traditional carbohydrate antigen 125 and human epididymal protein 4 serological tumor markers(AUC=0.862).Conclusion Tissue miR-142-3p and miR-598-3p may be useful biomarkers that can enhance the value of serum CA125 and HE4 in differentiating EOC from benign ovarian tumors.
NETL
NSTL
万方数据
