Effect of poric acid in polycystic ovary syndrome rats by regulating the Hippo-YAP signaling pathway
Objective To investigate the protective effect of poric acid in polycystic ovary syndrome(PCOS)rats by regulating the Hippo-Yes associated protein(YAP)signaling pathway.Methods Rats were divided into blank group,model group,metformin group(100 mg/kg),poric acid low(10 mg/kg),high dose group(40 mg/kg),PY-60 group(YAP activator PY-60 10 μmol/L),and high-dose poric acid+PY-60 group(40 mg/kg poric acid+10 μmol/L PY-60),with 10 rats in each group.After treatment,the body weight,FBG,and FINS levels of rats were detected,and the HOMA-IR was calculated.The patho-logical changes and apoptosis of ovarian tissue were observed;serum levels of FSH,LH,T,and E2 were detected;Western blot was used to detect Hippo-YAP signaling pathway-related protein expression in rat ovarian tissue.Results Compared with the blank group,the number of days between estrus,body weight,FBG,FINS,homeostatic model assessment-insulin resis-tance(HOMA-IR),cell apoptosis rate,number of cystic follicles,the levels of T,LH/FSH,LH,and the protein expression of phosphorylated(p)-YAP,p-the transcriptional co-activator with PDZ-binding motif(TAZ)in the model group were obviously increased,the E2,FSH,and corpus luteum count were obviously decreased(P＜0.05).Compared with the model group,the change trend of corresponding indexes in the low dose,high dose and metformin groups was opposite to the above.PY-60 alleviated the effect of poric acid in improving ovarian damage in PCOS rats.Conclusion Poric acid may improve ovarian damage in PCOS rats by inhibiting the Hippo-YAP pathway.
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