Effects of sufentanil on proliferation,migration,and invasion of ovarian cancer by regulating the Fas/FasL signaling pathway
Objective To investigate the effects of sufentanil on the proliferation,migration,and invasion of ovarian cancer cells,and to analyze whether its mechanism is related to the fatty acid synthetase(Fas)/fatty acid synthetase ligand(FasL)signaling pathway.Methods SKOV3 ovarian cancer cells were treated with 0.001-100 μmol/L sufentanil to screen for the optimal drug concentration.Ovarian cancer cell SKOV3 was separated into a normal control group(Control group),a low concentration sufentanil group(sufentanil-L group,0.01 μmol/L),a medium concentration sufentanil group(sufentanil-M group,0.1 μmol/L),a high concentration sufentanil group(sufentanil-H group,1 μmol/L),sufentanil-H+sh-NC group,and sufentanil-H+sh-Fas group.Proliferation,apoptosis,migration,and invasion abilities of SKOV3 cells were examined in each group.Western blot was applied to detect the expression of E-cadherin,N-cadherin,Snail,Fas,and FasL proteins.Results After treatment with sufentanil ranging from 0.001 to 100 μmol/L,the survival rate of ovarian cancer cells obviously decreased,in a concentration dependent,sufentanil with concentrations of 0.01,0.1,and 1 μmol/L was selected for subsequent experiments.Compared with the Control group,the number of clones formed,scratch healing rate,number of invasive cells,N-cadherin,and Snail protein levels in the sufentanil-L,sufentanil-M,and sufentanil-H groups were obviously reduced(P＜0.05),the apoptosis rate,E-cadherin,Fas,and FasL protein levels were obviously increased(P＜0.05).Inhibition of Fas expression at-tenuated the ability of sufentanil to inhibit cell proliferation,migration and invasion.Conclusion Sufentanil may inhibit the proliferation,migration,and invasion of ovarian cancer cells and promote apoptosis by activating the Fas/FasL signaling pathway.
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