Effects of salidroside on myocardial injury in gestational diabetes mellitus rats by regulating AGE-RAGE signaling pathway
Objective To investigate the effect of salidroside(Sal)on myocardial injury in gestational diabetes mel-litus rats by regulating the dvanced glycation end products(AGE)-receptor for advanced glycation end-products(RAGE)pathway.Methods Ten healthy pregnant mice were randomly regarded as the healthy group.The GDM rat model was ran-domly separated into GDM group(gavage of physiological saline),L-Sal group(gavage of 25 mg/kg Sal),H-Sal group(ga-vage of 100 mg/kg Sal),AGE group(gavage of 100 mg/kg Sal+tail vein injection of 10 mg/kg AGE serum protein),and FBS-ZMI group(gavage of 100 mg/kg Sal+intraperitoneal injection of 150 mg/kg FBS-ZMI).The healthy group was gavage of physiological saline.Ultrasound imaging was applied to analyze left ventricular ejection fraction(LVEF),left ventricular short axis fraction shorting(FS),left ventricular end diastolic diameter(LVEDD),and left ventricular end systolic diameter(LVESD).The levels of creatine kinase isoenzyme(CK-MB),cardiac troponin Ⅰ(cTnⅠ)and N-terminal pro-brain natriuretic peptide(NT-proBNP)were detected by automatic biochemical analyzer,and heart mass index(HWI)was calculated.H-E staining and Masson staining were applied to observe the morphology of myocardial tissue.Enzyme linked immunosorbent assay(ELISA)was applied to detect levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),IL-10,malondialdehyde(MDA),glutathione(GSH),and superoxide dismutase(SOD).Ferrous zinc colorimetric method was applied to analyze the total iron ion content in tissues.Immunoblotting was applied to determine the expression of AGE,RAGE,glutathione peroxi-dase 4(GPX4),acyl-CoA synthetase 4(ACSL4),and Bc12 proteins in tissues.Results Compared with the healthy group,the pathological changes of myocardial tissue in the GDM group were aggravated,the fasting blood glucose,LVEDD,LVESD,HWI,collagen volume fraction(CVF),CK-MB,cTnⅠ,NT-proBNP,total iron ion content,TNF-α,IL-6,MDA levels,and AGE,RAGE,ACSL4 expression increased,the LVEF,FS,IL-10,GSH,SOD levels,and the GPX4 and Bcl2 expression decreased(P<0.05).Compared with the GDM group,the pathological changes in myocardial tissue were reduced in the L-Sal and H-Sal groups,the LVEF,FS,IL-10,GSH,SOD levels and GPX4,Bcl2 expression increased,the fasting blood glucose,LVEDD,LVESD,HWI,CVF,CK-MB,cTnⅠ,NT-proBNP,total iron ion content,TNF-α,IL-6,MDA levels,and AGE,RAGE,ACSL4 expression reduced(P<0.05).Compared with the H-Sal group,the AGE group was able to reverse the changes in the above indicators and exacerbate the pathological changes in myocardial tissue.FBS-ZMI group could enhance the changes in the above indicators and alleviate pathological changes in myocardial tissue.Conclusion Sal may inhibit the AGE-RAGE pathway and alleviate myocardial injury in GDM rats.
salidrosideadvanced glycation end product-receptor for advanced glycation end product pathwayges-tational diabetes mellitus ratsmyocardial injury